腺苷單磷酸活化蛋白激酶α1/AMPK α 1抗體品牌 英文名稱  Anti-AMPK alpha-1

中文名稱  腺苷單磷酸活化蛋白激酶α1/AMPK α 1抗體品牌 

別    名 5 AMP activated protein kinase alpha 1catalytic subunit; 5 AMP activated protein kinase catalytic alpha 1 chain; 5' AMP activated protein kinase catalytic subunit alpha 1; AAPK1; acetyl CoA carboxylase kinase; AI194361; AI450832; AL024255; AMP -activate kinase alpha 1 subunit; AMP-activated protein kinase, catalytic, alpha -1; AMPK 1; AMPK alpha 1 chain; AMPK; AMPK1; AMPKa1; AMPKalpha1; C130083N04Rik; cb116; EC 2.7.11.1; HMG CoA reductase kinase;hormone sensitive lipase kinase; im:7154392; kinase AMPK alpha1; MGC33776; MGC57364; PRKAA 1; PRKAA1; Protein kinase AMP activated alpha 1 catalytic subunit; SNF1-like protein AMPK; wu:fa94c10.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 神經(jīng)生物學(xué) 激酶和磷酸酶 Alzheimer's

蛋白分子量  predicted molecular weight: 60kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human AMPK alpha 1 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

腺苷單磷酸活化蛋白激酶α1/AMPK α 1抗體品牌 產(chǎn)品應(yīng)用   WB=1:100-500 IHC-P=1:100-500 IHC-F=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq].

Function : Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Regulates lipid synthesis by phosphorylating and inactivating lipid metabolic enzymes such as ACACA, ACACB, GYS1, HMGCR and LIPE; regulates fatty acid and cholesterol synthesis by phosphorylating acetyl-CoA carboxylase (ACACA and ACACB) and hormone-sensitive lipase (LIPE) enzymes, respectively. Regulates insulin-signaling and glycolysis by phosphorylating IRS1, PFKFB2 and PFKFB3. AMPK stimulates glucose uptake in muscle by increasing the translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane, possibly by mediating phosphorylation of TBC1D4/AS160. Regulates transcription and chromatin structure by phosphorylating transcription regulators involved in energy metabolism such as CRTC2/TORC2, FOXO3, histone H2B, HDAC5, MEF2C, MLXIPL/ChREBP, EP300, HNF4A, p53/TP53, SREBF1, SREBF2 and PPARGC1A. Acts as a key regulator of glucose homeostasis in liver by phosphorylating CRTC2/TORC2, leading to CRTC2/TORC2 sequestration in the cytoplasm. In response to stress, phosphorylates 'Ser-36' of histone H2B (H2BS36ph), leading to promote transcription. Acts as a key regulator of cell growth and proliferation by phosphorylating TSC2, RPTOR and ATG1: in response to nutrient limitation, negatively regulates the mTORC1 complex by phosphorylating RPTOR component of the mTORC1 complex and by phosphorylating and activating TSC2. In response to nutrient limitation, promotes autophagy by phosphorylating and activating ULK1. AMPK also acts as a regulator of circadian rhythm by mediating phosphorylation of CRY1, leading to destabilize it. May regulate the Wnt signaling pathway by phosphorylating CTNNB1, leading to stabilize it. Also has tau-protein kinase activity: in response to amyloid beta A4 protein (APP) exposure, activated by CAMKK2, leading to phosphorylation of MAPT/TAU; however the relevance of such data remains unclear in vivo. Also phosphorylates CFTR, EEF2K, KLC1, NOS3 and SLC12A1.

Subunit : AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2.

Subcellular Location : Cytoplasm. Nucleus. Note=In response to stress, recruited by p53/TP53 to specific promoters.

Phosphorylated at Thr-183 by STK11/LKB1 in complex with STE20-related adapter-alpha (STRADA) pseudo kinase and CAB39. Also phosphorylated at Thr-183 by CAMKK2; triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. CAMKK1 can also phosphorylate Thr-183, but at much lower lvel. Dephosphorylated by protein phosphatase 2A and 2C (PP2A and PP2C). Phosphorylated by ULK1 and ULK2; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK.

Similarity : Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.

Contains 1 protein kinase domain.

Database links : UniProtKB/Swiss-Prot: Q13131.4

AMPKα1 (AMP-activated Protein Kinase-α1)（腺苷單磷酸活化蛋白激酶-α1）是一種參與細(xì)胞適應(yīng)能量危機(jī)的應(yīng)激反應(yīng)酶,AMPK不僅可以在細(xì)胞水平作為能量的感受器，還可以通過激素和細(xì)胞因子，如瘦素、脂聯(lián)素和ghrelin來參與調(diào)節(jié)機(jī)體的能量消耗和能量攝入.

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

腺苷單磷酸活化蛋白激酶α1/AMPK α 1抗體品牌 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

Anti-CCL9/CCL10/MIP-1 Gamma/FITC 熒光素標(biāo)記巨噬細(xì)胞蛋白-1γ抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-HER3/ErbB3 /FITC 熒光素標(biāo)記HER3受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CDO1 半胱氨酸雙加氧酶1抗體 規(guī)格 0.2ml

SOD2 (superoxide-dimutase-2) 超氧化物歧化酶2抗原 0.5ml

IDH1 英文名稱： 異檸檬酸脫氫酶1抗體 0.2ml

Rhesus antibody Rh Thrap3/TRAP150 甲狀腺激素受體相關(guān)蛋白3抗體 規(guī)格 0.2ml

Anti-HER3/ErbB3 /FITC 熒光素標(biāo)記HER3受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Human small breast epithelial mucin,SBEM ELISA Kit 人小表皮粘蛋白Multi-class antibodies規(guī)格： 48T

Anti-Phospho-SHIP2 (Tyr542) 磷酸化蛋白酪氨酸磷酸酶2抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Goat Anti-rat IgG/PE-CY5 PE-CY5標(biāo)記的羊抗大鼠IgG 規(guī)格 0.1ml

DHEA(Mouse Dehydroepiandrosterone) ELISA Kit 小鼠脫氫表雄酮 96T

phospho-NPTX1(Tyr344) 英文名稱： 磷酸化神經(jīng)細(xì)胞正五聚體1抗體 0.1ml

Beta-synuclein 英文名稱： 核突觸蛋白β抗體 0.2ml

Anti-Phospho-SHIP2 (Tyr542) 磷酸化蛋白酪氨酸磷酸酶2抗體Multi-class antibodies規(guī)格： 0.1ml

Mouse keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit 小鼠角化細(xì)胞因子/雙調(diào)蛋白Multi-class antibodies規(guī)格： 48T

Anti-BSP/FITC 熒光素標(biāo)記骨涎蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh EpCAM/CD326 上皮特異性抗原抗體 規(guī)格 0.1ml

D2D (Rabbit D-Dimer) ELISA Kit 兔子D二聚體 96T

phospho-p38 MAPK (Ty*3) 英文名稱： 磷酸化p38MAPK抗體 0.1ml

APPBP2 英文名稱： β淀粉樣蛋白前體蛋白結(jié)合蛋白2抗體 0.2ml

Anti-BSP/FITC 熒光素標(biāo)記骨涎蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

CL-0159MLTC-1(小鼠間質(zhì)細(xì)胞瘤細(xì)胞)5×106cells/瓶×2

ASGR1 Others Human 人 ASGPR1 / ASGR1 人細(xì)胞裂解液 (陽性對(duì)照)

小鼠肝實(shí)質(zhì)細(xì)胞完全培養(yǎng)基 100mL

CT26.WT小鼠細(xì)胞 CT26.WT mouse colon cancer cells DMEM培養(yǎng)基+10%FBS

LIF Protein Human 重組人 LIF 蛋白 (His 標(biāo)簽)

RKO-AS45-1(人轉(zhuǎn)基因細(xì)胞) 5×106cells/瓶×2 6T-CEM(T細(xì)胞)

CL-0156MFC(小鼠細(xì)胞)5×106cells/瓶×2

CD83 Others Cynomolgus 食蟹猴 CD83 人細(xì)胞裂解液 (陽性對(duì)照)

人*細(xì)胞裂解物HHL

UACC812細(xì)胞，人導(dǎo)管瘤細(xì)胞 鼠腹水瘤,EAC-E2G8細(xì)胞 腦動(dòng)脈平滑肌細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

BEL-7405(人細(xì)胞) 5×106cells/瓶×2

HPASMC Pellet 人肺動(dòng)脈平滑肌細(xì)胞團(tuán)塊 > 1 mio.cells 人*細(xì)胞裂解物HHL

腺苷單磷酸活化蛋白激酶α1/AMPK α 1抗體品牌 馬間葉干細(xì)胞(脂肪)(5×105)

人腎小球內(nèi)皮細(xì)胞RNAHRGEC miRNA5 μg

牛源細(xì)胞

C57小鼠瘤株;ME (Me) 小鼠真皮成纖維細(xì)胞完全培養(yǎng)基 100mL

人絨毛膜間充質(zhì)基質(zhì)細(xì)胞 Human

TNFRSF14 Others Human 人 TNFRSF14 / HVEA / HVEM 人細(xì)胞裂解液 (陽性對(duì)照)

技術(shù)外包服務(wù):

★腺苷單磷酸活化蛋白激酶α1/AMPK α 1抗體品牌 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。