產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-PSCA 

中文名稱   干細(xì)胞抗原抗體品牌 

別    名  prostate stem cell antigen preproprotein; PRO 232; PRO232; Prostate stem cell antigen; PSCA_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg 1ml/1mg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 干細(xì)胞 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 11kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PSCA C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

干細(xì)胞抗原抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene has a nonsynonymous nucleotide polymorphism at its start codon. This gene encodes a glycosylphosphatidylinositol-anchored cell membrane glycoprotein. In addition to being highly expressed in the prostate it is also expressed in the bladder, placenta, colon, kidney, and stomach. This gene is up-regulated in a large proportion of prostate cancers and is also detected in cancers of the bladder and pancreas. This gene has a nonsynonymous nucleotide polymorphism at its start codon.

Function : May be involved in the regulation of cell proliferation. Has a cell-proliferation inhibition activity in vitro.

Subcellular Location : Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity : Highly expressed in prostate (basal, secretory and neuroendocrine epithelium cells). Also found in bladder (transitional epithelium), placenta (trophoblasts), stomach (neuroendocrine cells), colon (neuroendocrine cells) and kidney (collecting ducts). Overexpressed in prostate cancers and expression is correlated with tumor stage, grade and androgen-independence. Highly expressed in prostate cancer bone metastases. Expressed in gastric epithelial cells, mainly in the isthmus (at protein level). Not detected in normal intestinal epithelium (at protein level).

Database links :

UniProtKB/Swiss-Prot: O43653.1

Entrez Gene: 8000 Human

Omim: 602470 Human

SwissProt: O43653 Human

Unigene: 652235 Human

干細(xì)胞抗原，除外，尿道、胃等多種組織都分布。

Anti-CD229/SLAMF3/FITC 熒光素標(biāo)記CD229抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-IL-5/FITC 熒光素標(biāo)記白介素5抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CD244 CD244抗體 規(guī)格 0.2ml

MMP-10(matrix metalloproteinase 10) 基質(zhì)金屬蛋白酶-10抗原 0.5mg

ICOS/CD278 英文名稱： 誘導(dǎo)協(xié)同刺激分子CD278抗體 0.2ml

Rhesus antibody Rh STK36 絲氨酸/蘇氨酸激酶36融合同源蛋白抗體 規(guī)格 0.2ml

Anti-IL-5/FITC 熒光素標(biāo)記白介素5抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Ki-67/FITC 熒光素標(biāo)記Ki-67 Antigen抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-SirT1 (Ser47) /FITC 熒光素標(biāo)記磷酸化沉默調(diào)節(jié)蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ALPK3 α蛋白激酶3抗體(心肌) 規(guī)格 0.2ml

Rabbit Anti-human IgM/Alexa Fluor 555 Alexa Fluor 555標(biāo)記的兔抗人IgM 0.1ml

GPS2 英文名稱： G蛋白通路抑制物蛋白2抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-Guinea pig IgM/PE-Cy3 PE-Cy3標(biāo)記的兔抗豚鼠IgM 規(guī)格 0.1ml

Anti-Phospho-SirT1 (Ser47) /FITC 熒光素標(biāo)記磷酸化沉默調(diào)節(jié)蛋白1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

IDO(Human indoleamine 2,3-dioxygenase) ELISA Kit 人吲哚胺2,3-雙加氧酶Multi-class antibodies規(guī)格： 48T

Anti-NDRG3 基因NDRG3抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh IL-33/NFHEV 白介素33抗體 規(guī)格 0.1ml

Urotensin II ELISA kit 大鼠尾加壓素2 96T

phospho-P53(Ser376) 英文名稱： 磷酸化抑制基因P53抗體 0.1ml

Contactin-1 英文名稱： 神經(jīng)細(xì)胞表面蛋白F3 0.2ml

Anti-NDRG3 基因NDRG3抗體Multi-class antibodies規(guī)格： 0.2ml

CL-0175OK(負(fù)鼠腎小管細(xì)胞)5×106cells/瓶×2

NRG1 Others Human 人 NRG1-beta 1 (ECD) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

小鼠腸動(dòng)脈內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

Beta-TC-6小鼠胰島素瘤胰島β細(xì)胞 Beta-TC-6 mouse insulinoma beta cell of islet DMEM培養(yǎng)基+15%熱滅活FBS

OSM Protein Mouse 重組小鼠 Oncostatin M / OSM 蛋白

RIN-m5f(大鼠胰島β細(xì)胞瘤細(xì)胞) 5×106cells/瓶×2 SGC7901(胃腺癌細(xì)胞)

CL-0172NR8383(大鼠肺泡巨噬細(xì)胞)5×106cells/瓶×2

PDCD1LG2 Others Cynomolgus 食蟹猴 B7-DC / PD-L2 / CD273 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人無(wú)色素睫狀上皮細(xì)胞裂解物HNPCEpiCL

M453細(xì)胞，人癌細(xì)胞 鼠細(xì)胞,S-180-S2D9細(xì)胞 組織源性原代細(xì)胞Many types of cells包裝：5 × 105次方(1ml)/1ml

B16-F10(小鼠皮膚細(xì)胞) 5×106cells/瓶×2

NHEM M2 adult Pellet 正常人表皮黑素細(xì)胞團(tuán)塊(捐獻(xiàn)者)，培養(yǎng)在M2培養(yǎng)液中 > 1 mio.cells 人腎小球內(nèi)皮細(xì)胞裂解物HRGECL

干細(xì)胞抗原抗體品牌 EPHB1 Others Human 人 EphB1 / EPHT2 (aa 565-984) 桿狀病毒-昆蟲細(xì)胞裂解液 (陽(yáng)性對(duì)照)

小鼠T淋巴細(xì)胞;Cl.Ly1+2-/9

SP2/0, 小鼠細(xì)胞

mEPC, 小鼠內(nèi)皮祖細(xì)胞-骨髓 小鼠精母細(xì)胞，GC-2spd細(xì)胞 Mv.1.Lu(NBL-7)(貂肺上皮細(xì)胞)

人類原巨核細(xì)胞型細(xì)胞;UT-7

CD200R4 Others Mouse 小鼠 CD200R4 / CD200 Receptor 4 / CD200RLa 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 干細(xì)胞抗原抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）干細(xì)胞抗原抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。