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蛋白質(zhì)磷酸酶-2A抗體規(guī)格 英文名稱  Anti-PP2A alpha/beta

中文名稱  蛋白質(zhì)磷酸酶-2A抗體規(guī)格 

別    名 PPP2CA; PP2A alpha;PP-2A; PP2A-Cα/β; PP2A C; PP2Ac; PP2CA; PPP2CA; Protein phosphatase 2, catalytic subunit, alpha isoform; Replication protein C; RP C; PP2AA_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學 信號轉(zhuǎn)導 細胞周期蛋白 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 34kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PP-2A C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

蛋白質(zhì)磷酸酶-2A抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Kinases and Phosphatases

Serine/threonine protein phosphatase 2A , catalytic subunit, alpha isoform(APP-2A-alpha) (also designated Replication protein C, RP-C) can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. PP2A consists of a common heterodimeric core enzyme, composed of a 34 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. It belongs to the PPP phosphatase family. PP-1 subfamily.

This antibody is crosed with PP-2A subunit A,B,C.

Function : PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.

Subcellular Location : Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity).

Similarity : Belongs to the PPP phosphatase family. PP-1 subfamily.

Database links :

UniProtKB/Swiss-Prot: P67775.1

Entrez Gene: 1432 Human

Entrez Gene: 26416 Mouse

Entrez Gene: 81649 Rat

Omim: 600289 Human

SwissProt: Q16539 Human

SwissProt: P47811 Mouse

SwissProt: P70618 Rat

Unigene: 485233 Human

激酶和磷酸酶（Kinases and Phosphatases） PP-2A (protein phosphatase 2A catalytic subunit；PP2A alpha；)參與酵母細胞及兩棲類卵母細胞有絲分裂的蛋白絲/蘇氨酸磷酸酶。

此酶的表達與細胞周期調(diào)節(jié)有關(guān)。此抗體與PP-2A subunit A,B,C 均有交叉反應(yīng)。

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