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血小板源性生長因子-B抗體說明書 英文名稱  Anti-PDGF-B

中文名稱  血小板源性生長因子-B抗體說明書 

別    名 PDGF-B; Platelet-derived growth factor B-chain; PDGF AB + BB; Becaplermin; c sis; FLJ1285; Oncogene SIS; PDGF 1; PDGF 2; PDGF A; PDGF A chain; PDGF B chain; PDGF subunit A; PDGF subunit B; PDGF1; PDGF2; PDGFA; PDGFB; PDGFR1; Platelet derived growth factor 2; Platelet derived growth factor alpha; Platelet derived growth factor alpha chain; Platelet derived growth factor alpha polypeptide; Platelet derived growth factor B chain; Platelet derived growth factor beta; Platelet derived growth factor beta polypeptide; Platelet derived growth factor subunit A; Platelet derived growth factor subunit B; SIS; SSV; v sis platelet derived growth factor beta polypeptide.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep

產(chǎn)品類型   一抗  

研究領(lǐng)域    心 信號(hào)轉(zhuǎn)導(dǎo) 生長因子和激素

蛋白分子量  predicted molecular weight: 12kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PDGF-B

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

血小板源性生長因子-B抗體說明書 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heal the wound. [SUBUNIT] Antiparallel disulfide-linked dimer of nonidentical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. Belongs to the PDGF/VEGF growth factor family.

Function : Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.

Subunit : Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1.

Subcellular Location : Secreted. Note=Released by platelets upon wounding.

DISEASE : Note=A chromosomal aberration involving PDGFB is found in dermatofibrosarcoma protuberans. Translocation t(17;22)(q22;q13) with PDGFB.

Similarity : Belongs to the PDGF/VEGF growth factor family.

Database links : Entrez Gene: 5155 Human

Entrez Gene: 18591 Mouse

Entrez Gene: 24628 Rat

Omim: 190040 Human

SwissProt: P01127 Human

SwissProt: P31240 Mouse

SwissProt: Q05028 Rat

Unigene: 1976 Human

生長因子和激素（ Growth Factor and Hormones）

PDGF（platelet-dirived growth factor）是一種堿性蛋白，分為兩個(gè)亞基（A、B鏈）通過二硫鍵結(jié)合而成的兩條多肽鏈的異二聚體，分子量為28-35kDa。為促細(xì)胞分裂劑，可使小鼠成纖維細(xì)胞及其他多種細(xì)胞增殖。*研究發(fā)現(xiàn)了PDGF家族兩種新的基因PDGF-C、PDGF-D。

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