產品訂購信息：
英文名稱  Anti-PMS/FOLH1/PMSA 

中文名稱   特異膜抗原抗體規(guī)格 

別    名  FOLH; PMSA; Cell growth inhibiting protein 27; FGCP; Folate hydrolase 1; folate hydrolase; Folate hydrolase prostate specific membrane antigen 1; FOLH 1; FOLH; FOLH1; Folylpoly gamma glutamate carboxypeptidase; GCP 2; GCP II; GCP2; GCPII; glutamate carboxylase II; Glutamate carboxypeptidase 2; glutamate carboxypeptidase II; Membrane glutamate carboxypeptidase; mGCP; N acetylated alpha linked acidic dipeptidase 1; N acetylated alpha linked acidic dipeptidase I; NAALAD 1; NAALAD1; NAALAdase; NAALADase I; prostate specific membrane antigen; PSM; pteroylpoly gamma glutamate carboxypeptidase.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig 

產品類型   一抗  

研究領域     免疫學 細胞表面分子

蛋白分子量  predicted molecular weight: 84kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PMSA 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

特異膜抗原抗體規(guī)格 產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產品介紹 PMSA (FOLH1) is a type II transmembrane glycoprotein belonging to the M28 peptidase family. Three functionally distinct proteins are encoded, including folylpoly-gamma-glutamate carboxypeptidase in the intestine, N-acetylated alpha-linked acidic dipeptidase 1 in the brain, and prostate-specific membrane antigen in the prostate. A mutation in the intestinal form may be associated with impaired intestinal absorption of dietary folates, resulting in low blood folate levels and consequent hyperhomocysteinemia. The form expressed in the brain may be involved in a number of pathological conditions associated with glutamate excitotoxicity. The prostate form is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer. This gene likely arose from a duplication event of a nearby chromosomal region. Alternative splicing gives rise to multiple transcript variants.

Subunit : Homodimer.

Subcellular Location : Cell membrane; Single-pass type II membrane protein. Isoform PSMA': Cytoplasm.

Tissue Specificity : Highly expressed in prostate epithelium. Detected in urinary bladder, kidney, testis, ovary, fallopian tube, breast, adrenal gland, liver, esophagus, stomach, small intestine, colon and brain (at protein level). Detected in the small intestine, brain, kidney, liver, spleen, colon, trachea, spinal cord and the capillary endothelium of a variety of tumors. Expressed specifically in jejunum brush border membranes. In the brain, highly expressed in the ventral striatum and brain stem. Also expressed in fetal liver and kidney. Isoform PSMA' is the most abundant form in normal prostate. Isoform PSMA-1 is the most abundant form in primary prostate tumors. Isoform PSMA-2 is also found in normal prostate as well as in brain and liver. Isoform PSMA-9 is specifically expressed in prostate cancer.

Anti-ets1/E26 /FITC 熒光素標記Ets轉錄因子家族ets1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-CHRNA4/FITC 熒光素標記型乙酰膽堿受體α4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh C17orf77 17號染色體開放閱讀框77抗體 規(guī)格 0.2ml

RAR-Alpha (Retinoic acid -R-Alpha) 維受體-α 多肽 0.5mg

HTRA2 英文名稱： 線粒體絲酸蛋白酶抗體 0.2ml

Rhesus antibody Rh Ron 原癌基因c-Met相關酪氨酸激酶抗體 規(guī)格 0.1ml

Anti-CHRNA4/FITC 熒光素標記型乙酰膽堿受體α4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

anti-LH/CG Receptor/FITC 熒光素標記人促黃體生成素受體抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-Stat4 (Tyr693) /FITC 熒光素標記磷酸化信號轉導和轉錄激活因子4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh AF 4 protein 原癌基因AF4蛋白抗體 規(guī)格 0.2ml

Rabbit Anti-Goat IgM/PE PE標記的兔抗羊IgM 0.1ml

GIPC-2 英文名稱： 胞漿區(qū)相關蛋白GIPC2抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-dog IgG/PE PE標記的兔抗狗IgG 規(guī)格 0.1ml

Anti-Phospho-Stat4 (Tyr693) /FITC 熒光素標記磷酸化信號轉導和轉錄激活因子4抗體IgGMulti-class antibodies規(guī)格： 0.2ml

BetaGAL(Human Alpha-galactosidase) ELISA Kit 人β半乳糖苷酶Multi-class antibodies規(guī)格： 48T

Anti-NKA 神經激肽A抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh IL-13Ra1 白細胞介素-13受體a1抗體 規(guī)格 0.1ml

BGP/Gehrelin(Mouse brain-gut peptides) ELISA Kit 小鼠腦腸肽 96T

phospho-PARK2(Ser101) 英文名稱： 磷酸化帕金蛋白抗體 0.1ml

Complement C3 英文名稱： 補體C3a過敏毒素抗體 0.1ml

Anti-NKA 神經激肽A抗體Multi-class antibodies規(guī)格： 0.2ml

CL-0248ZR-75-30(人癌細胞)5×106cells/瓶×2

OXSR1 Others Human 人 OXSR1 / OSR1 桿狀病毒-昆蟲細胞裂解液 (陽性對照)

人腎近曲小管上皮細胞cDNAHRPTEpiC cDNA

SHZ-88細胞，大鼠癌細胞 人(轉染TK基因的GLC-82細胞),GLC-82-TK細胞 肺泡上皮細胞Many types of cells包裝：5 × 105次方(1ml)

犬胸腺細胞;cf2Th

TNFRSF10D Others Human 人 AILR4 / TNFRSF10D / DCR2 / CD264 人細胞裂解液 (陽性對照)

CL-0247ZR-75-1(人癌細胞)5×106cells/瓶×2

NRG1 Others Human 人 NRG1-alpha (ECD) 人細胞裂解液 (陽性對照)

小鼠甲狀腺上皮細胞完全培養(yǎng)基 100mL

Sf9昆蟲卵巢細胞 Sf9 insect ovarian cells GPM-115無血清無蛋白昆蟲細胞懸浮培養(yǎng)基(金普諾安 Cat#GPM001L01)

IL1A Protein Human 重組人 IL-1 alpha / IL1A / IL1F1 蛋白

PC-3M-IE8(人高轉移細胞株) 5×106cells/瓶×2 Lec1(卵巢細胞)

特異膜抗原抗體規(guī)格 EGFR Others Mouse 小鼠 EGFR / HER1 / ErbB1 人細胞裂解液 (陽性對照)

T-108A膠原酶(含10mL酶解緩沖液)1mL

小鼠子宮成纖維細胞完全培養(yǎng)基 100mL

F9小鼠 F9 mouse teratoma cells DMEM培養(yǎng)基+10%FBS

IL10 Protein Human 重組人 IL10 / Ierleukin-10 蛋白 (His 標簽)

SUP-B15(人Ph+急淋細胞系) 5×106cells/瓶×2 SHZ-88(大鼠癌細胞)

抗體的生物素化標記實驗要點：

1. 特異膜抗原抗體規(guī)格 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質之間的分子比按蛋白質表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結合產生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）特異膜抗原抗體規(guī)格 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結合位點與抗原決定簇之間立體構型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。