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泛素連接酶抗體規(guī)格 英文名稱  Anti-PIRH2

中文名稱  泛素連接酶抗體規(guī)格 

別    名 Androgen receptor N terminal interacting protein; Androgen receptor N-terminal-interacting protein; ARNIP; CH-rich-interacting match with PLAG1; CHIMP; E3 ubiquitin-protein ligase Pirh2; hARNIP; hPirh2; p53 induced protein with a RING H2 domain; p53-induced RING-H2 protein; PIRH2E; PIRH2F; PRO1996; RCHY1; Ring finger and CHY zinc finger domain containing 1 E3 ubiquitin protein ligase; RING finger and CHY zinc finger domain-containing protein 1; RING finger protein 199; RNF199; ZFP 363; Zinc finger protein 363; ZN363_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Cow, Horse

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 泛素

蛋白分子量  predicted molecular weight: 30kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human PIRH2 C-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

泛素連接酶抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 PirH2 has ubiquitin-protein ligase activity. It binds to p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53 (referenced from entrez gene).[SUBUNIT] Monomer and homodimer. [SUBCELLULAR LOCATION] Nucleus. Nucleus speckle. Cytoplasm.

Function : Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and CDKN1B. Preferentially acts on tetrameric p53/TP53. Increases AR transcription factor activity (By similarity). Contributes to the regulation of CDKN1B and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression.

Subunit : Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, CDKN1B, COPE, UBE2D2 and GORAB/NTKLBP1 (By similarity).

Subcellular Location : Nucleus (By similarity). Nucleus speckle (By similarity). Cytoplasm (By similarity).

Post-translational modifications : Subject to ubiquitination and proteasomal degradation (By similarity).

Similarity : Contains 1 CHY-type zinc finger.

Contains 1 CTCHY-type zinc finger.

Contains 1 RING-type zinc finger.

Database links : UniProtKB/Swiss-Prot: Q96PM5.1

Pirh2通過負(fù)反饋調(diào)控p53的轉(zhuǎn)錄激活和細(xì)胞生長；

Pirh2蛋白是一個(gè)新發(fā)現(xiàn)的泛素蛋白連接酶，受到p53的誘導(dǎo)調(diào)控表達(dá)，可促進(jìn)p53蛋白的泛素化降解。目前多用于的研究。

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