產(chǎn)品訂購(gòu)信息：
英文名稱  Anti-PLAUR/CD87 

中文名稱   尿激酶型纖溶酶原激活因子受體抗體品牌 

別    名  Plasminogen activator,urokinase receptor; Urokinase plasminogen activator surface receptor; Monocyte activation antigen Mo3; uPAR; U-PAR; MO3; UPAR; CD 87; CD87; CD87 antigen; MO 3; MO3; Monocyte activation antigen Mo3; Plasminogen activator urokinase receptor; PLAUR; U PAR; u plasminogen activator receptor; UPA receptor; UPAR; Urinary plasminogen activator receptor; URKR; Urokinase plasminogen activator receptor; Urokinase plasminogen activator surface receptor.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來(lái)源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域     心 細(xì)胞生物 免疫學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞膜受體

蛋白分子量  predicted molecular weight: 31kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PLAUR C-terminus 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

尿激酶型纖溶酶原激活因子受體抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes the receptor for urokinase plasminogen activator and, given its role in localizing and promoting plasmin formation, likely influences many normal and pathological processes related to cell-surface plasminogen activation and localized degradation of the extracellular matrix. It binds both the proprotein and mature forms of urokinase plasminogen activator and permits the activation of the receptor-bound pro-enzyme by plasmin. The protein lacks transmembrane or cytoplasmic domains and may be anchored to the plasma membrane by a glycosyl-phosphatidylinositol(GPI) moiety following cleavage of the nascent polypeptide near its carboxy-terminus. However, a soluble protein is also produced in some cell types. Alternative splicing results in multiple transcript variants encoding different isoforms. The proprotein experiences several post-translational cleavage reactions that have not yet been fully defined. [provided by RefSeq].

Function : Acts as a receptor for urokinase plasminogen activator. Plays a role in localizing and promoting plasmin formation. Mediates the proteolysis-independent signal transduction activation effects of U-PA. It is subject to negative-feedback regulation by U-PA which cleaves it into an inactive form.

Subcellular Location : Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 2: Secreted (Probable).

Tissue Specificity : Expressed in neurons of the rolandic area of the brain (at protein level). Expressed in the brain.

Anti-CLEC-2/CLEC1B/FITC 熒光素標(biāo)記C型凝集素結(jié)構(gòu)域家族1成員B抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-IRS-3/FITC 熒光素標(biāo)記受體底物-3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CCDC93 卷曲螺旋結(jié)構(gòu)域蛋白93抗體 規(guī)格 0.2ml

HtrA2/Omi peptide 絲酸蛋白酶/線粒體絲酸蛋白酶多肽抗原 0.5mg

HSJ1 英文名稱： 熱休克蛋白J1抗體 0.2ml

Rhesus antibody Rh SORBS2/ArgBP2 精氨酸結(jié)合蛋白2抗體 規(guī)格 0.2ml

Anti-IRS-3/FITC 熒光素標(biāo)記受體底物-3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-LRRK2 /FITC 熒光素標(biāo)記富亮氨酸重復(fù)激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-TAK1 (Thr187)/FITC 熒光素標(biāo)記磷酸化轉(zhuǎn)化生長(zhǎng)因子β活化激酶1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ADCY7 腺苷酸環(huán)化酶7抗體 規(guī)格 0.2ml

Rabbit Anti-dog IgG/PE-CY5 PE-CY5標(biāo)記的兔抗狗IgG 0.1ml

GDF6 英文名稱： 生長(zhǎng)分化因子6抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-chicken IgM 兔抗雞IgM 規(guī)格 0.2ml

Anti-Phospho-TAK1 (Thr187)/FITC 熒光素標(biāo)記磷酸化轉(zhuǎn)化生長(zhǎng)因子β活化激酶1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

anti-RV(Human anti-rabies virus antibody) ELISA Kit 人抗毒抗體Multi-class antibodies規(guī)格： 48T

Anti-Phospho-IRF7 (Ser471/472) 磷酸化干擾素調(diào)節(jié)因子7抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh LGI4 含亮氨酸神經(jīng)失活蛋白4抗體 規(guī)格 0.1ml

HCG(Human Chorionic Gonadotrophin) ELISA Kit 人絨毛膜激素 96T

Phospho-PDPK1(Tyr373 + Tyr376) 英文名稱： 磷酸化3磷酸肌醇依賴性蛋白激酶1抗體 0.1ml

C11ORF46 英文名稱： 11號(hào)染色體開(kāi)放閱讀框46抗體 0.2ml

Anti-Phospho-IRF7 (Ser471/472) 磷酸化干擾素調(diào)節(jié)因子7抗體Multi-class antibodies規(guī)格： 0.1ml

CL-0303PATU8988(人*癌細(xì)胞)5×106cells/瓶×2

CD40LG Others Canine 狗 CD40L / CD154 / TNFSF5 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人平滑肌細(xì)胞總RNAHBVSMC tDNA

山羊皮膚成纖維樣細(xì)胞;DG-S1 胚腎細(xì)胞，AAV-293細(xì)胞 HP615細(xì)胞，615小鼠瘤株

人干細(xì)胞因子單克隆抗體細(xì)胞株;AMS2(SCF3)

HA Others H5N1 甲型 H5N1 (A/Common magpie/Hong Kong/2256/2006) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CL-0301M-NFS-60 (小鼠細(xì)胞G-CSF依賴性)5×106cells/瓶×2

HA Others H5N1 甲型 H5N1 (A/bar-headed goose/Qinghai/14/2008) 血凝素 (Hemagglutinin / HA) 桿狀病毒-昆蟲細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人臍動(dòng)脈平滑肌細(xì)胞cDNAHUASMC cDNA

V79細(xì)胞，中國(guó)倉(cāng)鼠肺細(xì)胞 人亞歷山大細(xì)胞系,PLC/PRF/5細(xì)胞 膀胱上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

猞猁皮膚成纖維樣細(xì)胞;ELS1

VCAM1 Others Human 人 CD106 / VCAM1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

尿激酶型纖溶酶原激活因子受體抗體品牌 TF Others Sus scrofa (Pig) 野豬 ansferrin / TF 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

MA, 小鼠星形膠質(zhì)細(xì)胞

大鼠小腦顆粒細(xì)胞完全培養(yǎng)基 100mL

SNU-638人細(xì)胞 Human gasic cancer cells SNU-638 1640+10% FBS

IFNB1 Protein Human 重組人 Ierferon beta / IFN-beta / IFNB 蛋白 (Fc 標(biāo)簽)

NCI-H2170(人肺鱗癌細(xì)胞) 5×106cells/瓶×2 WI-38(胚肺細(xì)胞)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 尿激酶型纖溶酶原激活因子受體抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）尿激酶型纖溶酶原激活因子受體抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。