磷酸化過(guò)氧化酶活化增生受體γ抗體說(shuō)明書(shū) 英文名稱  Anti-Phospho-PPAR Gamma (ser112)

中文名稱  磷酸化過(guò)氧化酶活化增生受體γ抗體說(shuō)明書(shū) 

別    名 CIMT1; HUMPPARG; NR1C3; Nuclear receptor subfamily 1 group C member 3; PAX8/PPARG Fusion Gene; Peroxisome Proliferator Activated Receptor gamma; PPAR gamma; PPARG; PPARG1; PPARG2; PPARG3l; PPARG_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Rabbit, Guinea Pig

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域     免疫學(xué) 細(xì)胞凋亡 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 57kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthesised phosphopeptide derived from human PPAR Gamma around the phosphorylation site of ser112

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化過(guò)氧化酶活化增生受體γ抗體說(shuō)明書(shū) 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. [provided by RefSeq, Jul 2008].

Function : Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.

Subunit : "Forms a heterodimer with the retinoic acid receptor RXRA called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with FAM120B. Interacts with PRDM16 (By similarity). Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 LXXLL motifs. Interacts with DNTTIP2, MAP2K1/MEK1, PRMT2 and TGFB1I1. Interacts with PDPK1. Interacts with ASXL1 AND ASXL2.

Subcellular Location : Nucleus. Cytoplasm.

DISEASE : Note=Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension. PPARG mutations may be associated with colon cancer.

Defects in PPARG may be associated with susceptibility to obesity (OBESITY) [MIM:601665]. It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat.

Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia.

Genetic variations in PPARG can be associated with susceptibility to glioma type 1 (GLM1) [MIM:137800]. Gliomas are central nervous system neoplasms derived from glial cells and comprise astrocytomas, glioblastoma multiforme, oligodendrogliomas, and ependymomas. Note=Polymorphic PPARG alleles have been found to be significantly over-represented among a cohort of American patients with sporadic glioblastoma multiforme suggesting a possible contribution to disease susceptibility.

Similarity : Belongs to the nuclear hormone receptor family. NR1 subfamily.

Contains 1 nuclear receptor DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: P37231.3

類固醇受體（Steroid Receptors）

過(guò)氧化物酶體增殖物激活受體γ（PPARγ）主要存在于白色脂肪組織，PPARγ對(duì)于脂肪生成、穩(wěn)定、反應(yīng)、和等的發(fā)生都起到重要的作用。主要在脂肪細(xì)胞內(nèi)表達(dá)。PPARγ是噻唑烷二酮類藥物（TZDs）作用的藥靶，又是脂肪細(xì)胞分化的重要調(diào)節(jié)因子。經(jīng)研究發(fā)現(xiàn)，PPARγ在及抵抗的機(jī)制中具有十分重要的意義，是的重要藥靶。

過(guò)氧化物酶體增殖物激活受體γ（PPARγ）屬Ⅱ型核受體超家族成員，主要在脂肪細(xì)胞內(nèi)表達(dá)。PPARγ是噻唑烷二酮類藥物（TZDs）作用的藥靶，又是脂肪細(xì)胞分化的重要調(diào)節(jié)因子?，F(xiàn)有研究（包括一次于美國(guó)加州大學(xué)進(jìn)行的研究）發(fā)現(xiàn)PPARγ在及抵抗的機(jī)制中具有十分重要的意義，是的重要藥靶。目前，該受體蛋白質(zhì)水平的篩選模式已經(jīng)建立，并正在建立該受體的報(bào)告基因的細(xì)胞水平篩選評(píng)價(jià)模式。

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

磷酸化過(guò)氧化酶活化增生受體γ抗體說(shuō)明書(shū) 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。

正在熱銷的相關(guān)產(chǎn)品：

Anti-cofilin/FITC 熒光素標(biāo)記兔抗人、大、小鼠絲切蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-ISR- Alpha protein/FITC 熒光素標(biāo)記受體-α蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CCDC88B/BRLZ/HkRP3 大腦亮氨酸拉鏈結(jié)構(gòu)域蛋白抗體 規(guī)格 0.2ml

HSTF2/HSF2 peptide 熱休克轉(zhuǎn)錄因子2抗原 0.5mg

HSD17B2 英文名稱： 羥類固醇脫氫酶17β2抗體 0.1ml

Rhesus antibody Rh SOD3 超氧化物歧化酶3抗體 規(guī)格 0.2ml

Anti-ISR- Alpha protein/FITC 熒光素標(biāo)記受體-α蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

ECP ELISA Kit 大鼠嗜酸性粒細(xì)胞陽(yáng)離子蛋白Multi-class antibodies規(guī)格： 48T

Anti-Phospho-TCTP (Ser46) 磷酸化翻譯控制蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh Goat Anti-Bovine IgG/HRP 辣根過(guò)氧化物酶標(biāo)記的羊抗IgG 規(guī)格 0.1ml

DA ELISA Kit 大鼠多巴胺 96T

Phospho-NFKB1 (Ser927) 英文名稱： 磷酸化細(xì)胞核因子p50/k基因結(jié)合核因子抗體 0.1ml

BLAME 英文名稱： BLAME抗體 0.1ml

Anti-Phospho-TCTP (Ser46) 磷酸化翻譯控制蛋白抗體Multi-class antibodies規(guī)格： 0.1ml

H2-K1(Human Histocompatibility 2-K1-K region) ELISA Kit 人組織相容性2-K1-K 區(qū)Multi-class antibodies規(guī)格： 48T

Anti-PAX5 配對(duì)盒基因5抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh HOMER3 HOMER3蛋白抗體 規(guī)格 0.2ml

Human anti-cartilage-antibody ELISA Kit 人抗軟骨抗體 96T

phospho-PI3-kinase regulatory subunit gamma (Tyr199) 英文名稱： 磷酸化磷脂酰肌醇激酶調(diào)節(jié)亞單位gamma抗體 0.1ml

Cullin 3 英文名稱： Cullin3抗體 0.2ml

Anti-PAX5 配對(duì)盒基因5抗體Multi-class antibodies規(guī)格： 0.2ml

CL-0326Caki-2(人腎透明細(xì)胞癌細(xì)胞)5×106cells/瓶×2

CD36 Others Cynomolgus 食蟹猴 CD36 / SCARB3 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人真皮成纖維細(xì)胞-新生兒總RNAHDF-n NA

MDA-MB-435細(xì)胞，人癌高轉(zhuǎn)移細(xì)胞 鼠癌細(xì)胞系,MA-782細(xì)胞 原代滑膜皮細(xì)胞特制基礎(chǔ)無(wú)血清培養(yǎng)基Many types of cells包裝：500/100ml

PATU8988(人*癌細(xì)胞) 5×106cells/瓶×2

HDLEC adult Pellet 人類皮膚淋巴管內(nèi)皮細(xì)胞團(tuán)塊(成年捐獻(xiàn)者) > 1 mio.cells 角質(zhì)細(xì)胞生長(zhǎng)添加物(不含BPE)KGS-2

CL-0324BT-20(人癌細(xì)胞)5×106cells/瓶×2

ROR1 Others Human 人 ROR1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人表皮黑色素細(xì)胞-深色素總RNAHEM-d NA

盤(pán)羊皮膚細(xì)胞;ASHS2 中國(guó)倉(cāng)鼠二氫葉酸還原酶缺陷細(xì)胞，CHO/dhFr-細(xì)胞 Ca763細(xì)胞，615小鼠株

轉(zhuǎn)化細(xì)胞

HA Others H5N1 甲型 H5N1 (A/Egypt/N05056/2009) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

磷酸化過(guò)氧化酶活化增生受體γ抗體說(shuō)明書(shū) TMED1 Others Human 人 TMED1 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人細(xì)胞;Hela S3 [HeLaS3]

人肝實(shí)質(zhì)細(xì)胞完全培養(yǎng)基 100mL

L6565細(xì)胞，小鼠克隆細(xì)胞系 破桿菌/梭菌Closidium tetani 猴脈絡(luò)膜-視網(wǎng)膜(內(nèi)皮)細(xì)胞;RF/6A

CXCL12 Protein Human 重組人 CXCL12 / SDF-1 蛋白 (isoform a, His 標(biāo)簽)

PIEC(豬髖動(dòng)脈內(nèi)皮細(xì)胞 ) 5×106cells/瓶×2 食蟹猴 CD10 / Neprilysin / MME 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

技術(shù)外包服務(wù):

★磷酸化過(guò)氧化酶活化增生受體γ抗體說(shuō)明書(shū) 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。