產(chǎn)品訂購信息：
英文名稱  Anti-Phospho-PEA15(Ser116) 

中文名稱   磷酸化星形膠質(zhì)細(xì)胞PEA15抗體品牌 

別    名  Astrocytic phosphoprotein PEA 15; Astrocytic phosphoprotein PEA15; HMAT 1; HMAT1; Homolog of mouse MAT 1 oncogene; Homolog of mouse MAT1 oncogene; HUMMAT 1H; HUMMAT1H; MAT 1; MAT 1H; MAT1; MAT1H; PEA 15; PEA-15； PEA15 protein; PED; Phosphoprotein enriched in astrocytes 15; Phosphoprotein enriched in astrocytes 15kD; Phosphoprotein enriched in diabetes; PEA15_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow, Rabbit 

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    免疫學(xué) 神經(jīng)生物學(xué) 信號(hào)轉(zhuǎn)導(dǎo) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 15kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human PEA15 around the phosphorylation site of Ser116 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

磷酸化星形膠質(zhì)細(xì)胞PEA15抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 PED/PEA 15 (Phosphoprotein Enriched in Diabetes/Phosphoprotein Enriched in Astrocytes 15 kDa) is a widely expressed 15 kDa protein comprised of an N terminal region containing a canonical Death Effector Domain (DED) sequence and a nuclear export signal, and a C terminal region containing two serine phosphorylation sites. PED/PEA 15 has been implicated in the regulation of multiple cellular processes including apoptosis, integrin activation, and insulin sensitive glucose transport in insulin responsive cells. Phosphorylation of both serine 104 (a Protein Kinase C site) and serine 116 (a substrate of CaMKII and Akt) is required for PED/PEA 15 function.

Function : Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface.

Subunit : Binds RPS6KA3, MAPK3 and MAPK1. Transient interaction with PLD1 and PLD2 (By similarity). Interacts with CASP8 and FADD.

Subcellular Location : Cytoplasm.

Post-translational modifications : Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones.

Similarity : Contains 1 DED (death effector) domain.

Database links : UniProtKB/Swiss-Prot: Q15121.2

Anti-Collagen X /FITC 熒光素標(biāo)記抗Ⅹ型膠原抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-PFK2/PFKFB3 /FITC 熒光素標(biāo)記6磷酸果糖激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CCDC40 卷曲螺旋結(jié)構(gòu)域蛋白40抗體 規(guī)格 0.2ml

Alpha-HCG(Human chorionic gonadotropin Alpha ) 人α亞基人絨毛膜激素多肽抗原 0.5mg

HSD17B1 英文名稱： 羥類固醇脫氫酶17β抗體 0.1ml

Rhesus antibody Rh SMMHC/Myosin 平滑肌肌球蛋白重鏈抗體 規(guī)格 0.1ml

Anti-PFK2/PFKFB3 /FITC 熒光素標(biāo)記6磷酸果糖激酶2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-MBP/FITC 熒光素標(biāo)記髓鞘堿性蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-Tie2 (Ser1119) /FITC 熒光素標(biāo)記磷酸化生成素受體2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ACVRL1/ALK1 激活素受體樣激酶1抗體 規(guī)格 0.2ml

Rabbit Anti-Bovine IgM/Gold 膠體金標(biāo)記的兔抗IgM 2ml

G 英文名稱： 延伸因子G2抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-Bov IgG/PE PE標(biāo)記的兔抗IgG 規(guī)格 0.1ml

Anti-Phospho-Tie2 (Ser1119) /FITC 熒光素標(biāo)記磷酸化生成素受體2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

ODF(Human osteoclast differentiation factor) ELISA Kit 人破骨細(xì)胞分化因子Multi-class antibodies規(guī)格： 48T

Anti-MCP-1/CCL2 單核細(xì)胞趨化蛋白1抗體(人)Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Kappa-casein/CSN3 κ-酪蛋白抗體 規(guī)格 0.2ml

E ELISA Kit 大鼠雌激素 96T

phospho-PIK3 gamma (Ser1101) 英文名稱： 磷酸化磷脂酰肌醇激酶PIK3-γ抗體 0.1ml

CEP135 英文名稱： 中心體蛋白135抗體 0.2ml

Anti-MCP-1/CCL2 單核細(xì)胞趨化蛋白1抗體(人)Multi-class antibodies規(guī)格： 0.2ml

CL-0329Chang liver (CCL13) (人張氏肝細(xì)胞)5×106cells/瓶×2

CNDP1 Others Mouse 小鼠 CNDP1 人細(xì)胞裂解液 (陽性對(duì)照)

小鼠肺動(dòng)脈成纖維細(xì)胞完全培養(yǎng)基 100mL

Psi2 DAP小鼠胚胎成纖維細(xì)胞 Psi2 DAP mouse embryo fibroblasts DMEM培養(yǎng)基+10%FBS

IL1B Protein Mouse 重組小鼠 IL-1 beta / IL1B 蛋白

NCI-H596(人肺腺鱗癌細(xì)胞) 5×106cells/瓶×2 A10(大鼠主動(dòng)脈平滑肌細(xì)胞)

CL-0326Caki-2(人腎透明細(xì)胞癌細(xì)胞)5×106cells/瓶×2

CD36 Others Cynomolgus 食蟹猴 CD36 / SCARB3 人細(xì)胞裂解液 (陽性對(duì)照)

人真皮成纖維細(xì)胞-新生兒總RNAHDF-n NA

MDA-MB-435細(xì)胞，人癌高轉(zhuǎn)移細(xì)胞 鼠癌細(xì)胞系,MA-782細(xì)胞 原代滑膜皮細(xì)胞特制基礎(chǔ)無血清培養(yǎng)基Many types of cells包裝：500/100ml

PATU8988(人*癌細(xì)胞) 5×106cells/瓶×2

HDLEC adult Pellet 人類皮膚淋巴管內(nèi)皮細(xì)胞團(tuán)塊(成年捐獻(xiàn)者) > 1 mio.cells 角質(zhì)細(xì)胞生長(zhǎng)添加物(不含BPE)KGS-2

磷酸化星形膠質(zhì)細(xì)胞PEA15抗體品牌 RSV-G Others Respiratory syncytial virus/RSV 人類合胞病毒 hRSV (A, rsb1734) glycoprotein G / RSV-G 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)

人髓核細(xì)胞裂解物HNPCL

小鼠肥大細(xì)胞瘤細(xì)胞;P815

KB細(xì)胞，口腔表皮樣癌細(xì)胞 人細(xì)胞,KYSE510細(xì)胞 家貓腎細(xì)胞;CATK1

tTA基因修飾的小鼠(B類);F9-CAG-tTA-3E5

HA Others H5N1 甲型 H5N1 (A/Vietnam/1194/2004) 血凝素 (Hemagglutinin / HA) 人細(xì)胞裂解液 (陽性對(duì)照)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化星形膠質(zhì)細(xì)胞PEA15抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）磷酸化星形膠質(zhì)細(xì)胞PEA15抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力。抗體的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。