產(chǎn)品訂購信息：
英文名稱  Anti-PARP1 

中文名稱   多腺苷二磷酸多聚酶抗體/多聚ADP-核糖聚合酶1費(fèi)用 

別    名  ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; PARP-1; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Cow 

產(chǎn)品類型   一抗  

研究領(lǐng)域    信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡

蛋白分子量  predicted molecular weight: 112kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PARP 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

多腺苷二磷酸多聚酶抗體/多聚ADP-核糖聚合酶1費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

Function : Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Subunit : Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Subcellular Location : Nucleus.

Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.

S-nitrosylated, leading to inhibit transcription regulation activity.

Similarity : Contains 1 BRCT domain.

Contains 1 PARP alpha-helical domain.

Contains 1 PARP catalytic domain.

Contains 2 PARP-type zinc fingers.

Database links : UniProtKB/Swiss-Prot: P09874.4

PARP(poly ADP-ribose polymerase/PARP-type 1)是DNA修復(fù)酶。

PARP是細(xì)胞凋亡核心成員半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA損傷修復(fù)與細(xì)胞凋亡中發(fā)揮著重要作用。Anti-PARP p85 是特意的PARPp85片段的特異抗體，由caspase剪切116kDa完整分子而得到的。

PARP是存在于多數(shù)真核細(xì)胞中的一個(gè)多功能蛋白質(zhì)翻譯后修飾酶。它通過識(shí)別結(jié)構(gòu)損傷的DNA片段而被激活，對(duì)聚腺苷二磷酸核糖聚合酶PARP被認(rèn)為是DNA損傷的感受器。它還能對(duì)許多核蛋白進(jìn)行聚腺苷二磷酸核糖基化。因此，在DNA損傷修復(fù)與細(xì)胞凋亡中發(fā)揮著重要作用，端錨聚合酶在癌細(xì)胞端粒結(jié)構(gòu)的調(diào)控機(jī)制中有重要作用。

Anti-phospho-GAB2 (Tyr452) /FITC 熒光素標(biāo)記磷酸化接頭蛋白Gab 2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-eNOS (Thr495)/FITC 熒光素標(biāo)記磷酸化合成酶3抗體(內(nèi)皮型)IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh BrdU 溴脫氧尿苷抗體 規(guī)格 0.2ml

AQP1(Aquaporin1) 水通道蛋白-1抗原 0.5mg

HS1BP3 英文名稱： 造血干細(xì)胞特異性相關(guān)結(jié)合蛋白3抗體 0.2ml

Rhesus antibody Rh RGS5 G蛋白信號(hào)轉(zhuǎn)導(dǎo)調(diào)節(jié)因子5抗體 規(guī)格 0.2ml

Anti-Phospho-eNOS (Thr495)/FITC 熒光素標(biāo)記磷酸化合成酶3抗體(內(nèi)皮型)IgGMulti-class antibodies規(guī)格： 0.2ml

TGF Beta2 ELISA Kit 大鼠轉(zhuǎn)化生長因子β2Multi-class antibodies規(guī)格： 48T

Anti-Phospho-TLK1 (Ser695) 磷酸化調(diào)節(jié)染色體組裝激酶1抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh GLUT8 葡萄糖轉(zhuǎn)運(yùn)蛋白8抗體 規(guī)格 0.2ml

CCSA-3(Human colon cancer-specific antigen-3) ELISA kit 人專一抗原3 96T

NKp80 英文名稱： 細(xì)胞毒性受體NK-p80抗體 0.2ml

ANTXR2 英文名稱： 炭疽毒素受體2抗體 0.2ml

Anti-Phospho-TLK1 (Ser695) 磷酸化調(diào)節(jié)染色體組裝激酶1抗體Multi-class antibodies規(guī)格： 0.1ml

26S PSM(Human 26S proteasome) ELISA Kit 人26S蛋白酶體Multi-class antibodies規(guī)格： 48T

Anti-NPM 核仁磷酸蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh IGSF5 免疫球蛋白超家族成員5抗體 規(guī)格 0.2ml

TRH(Mouse thyrotropin-releasing hormone) ELISA Kit 小鼠促甲狀腺素釋放激素 96T

phospho-PIK3R1(Tyr467) 英文名稱： 磷酸化磷脂酰肌醇激酶抗體 0.1ml

Alpha s1 Casein 英文名稱： α-s1酪蛋白抗體 0.2ml

Anti-NPM 核仁磷酸蛋白抗體Multi-class antibodies規(guī)格： 0.2ml

CL-0339G-401(人Wilms細(xì)胞)5×106cells/瓶×2

PHKG1 Others Human 人 PHKG1 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)

人食道平滑肌細(xì)胞總RNAHESMC NA

RD細(xì)胞，惡性胚胎橫紋肌瘤 人細(xì)胞系,Tul-1細(xì)胞 原代軟骨細(xì)胞特制基礎(chǔ)無血清培養(yǎng)基Many types of cells包裝：500/100ml

豹貓肺成纖維樣細(xì)胞;LCL2

HA Others H5N1 甲型 H5N1 (A/Anhui/1/2005) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽性對(duì)照)

CL-0338G1(發(fā)綠色熒光的小鼠胚胎干細(xì)胞)5×106cells/瓶×2

ESAM Others Mouse 小鼠 ESAM / Endothelial Cell Adhesion Molecule 人細(xì)胞裂解液 (陽性對(duì)照)

小鼠肺大動(dòng)脈內(nèi)皮細(xì)胞完全培養(yǎng)基 100mL

Eca-109人細(xì)胞 Human esophageal cancer cell line Eca-109. 1640+10% FBS

IL1B Protein Rat 重組大鼠 IL-1 beta / IL1B 蛋白 (mature form)

NCI-H524(人非小細(xì)胞細(xì)胞) 5×106cells/瓶×2 HUVEC(人臍內(nèi)皮細(xì)胞)

多腺苷二磷酸多聚酶抗體/多聚ADP-核糖聚合酶1費(fèi)用 DPYS Others Human 人 DPYS / Dihydropyrimidinase 桿狀病毒-昆蟲細(xì)胞裂解液 (陽性對(duì)照)

CM-H127人晶狀體上皮細(xì)胞完全培養(yǎng)基100mL

人軟骨細(xì)胞HC-a

羅猴胎腎細(xì)胞;MA104 腦細(xì)胞，C6細(xì)胞 NCI-H446 [H446](小細(xì)胞細(xì)胞)

人細(xì)胞;DU 145 [DU145;DU-145]

TNFRSF14 Others Mouse 小鼠 HVEM / TNFRSF14 人細(xì)胞裂解液 (陽性對(duì)照)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 多腺苷二磷酸多聚酶抗體/多聚ADP-核糖聚合酶1費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）多腺苷二磷酸多聚酶抗體/多聚ADP-核糖聚合酶1費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。