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神經(jīng)再生相關(guān)蛋白抗體規(guī)格 英文名稱  Anti-P311 protein

中文名稱  神經(jīng)再生相關(guān)蛋白抗體規(guī)格 

別    名 C5orf13; Neuronal protein 3.1; PRO1873; Protein p311; Chromosome 5 open reading frame 13; D4S114; PTZ17; NREP_HUMAN.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗  

研究領(lǐng)域     心 細(xì)胞生物 神經(jīng)生物學(xué) 細(xì)胞周期蛋白 細(xì)胞分化

蛋白分子量  predicted molecular weight: 7.9kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human P311 N-terminus

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

神經(jīng)再生相關(guān)蛋白抗體規(guī)格 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 P311, also called PTZ17, was identified by suppressive subtraction hybridization as potentially involved in smooth muscle (SM) myogenesis., an 8-kDa polypeptide, was previously shown to be highly expressed in invasive glioma cells. P311 is constitutively serine-phosphorylated; decreased phosphorylation is observed in migration-activated glioma cells. The primary amino acid sequence of P311 indicates a putative serine phosphorylation site (S59) near the PEST domain. Site-directed mutagenesis of S59A retarded P311 degradation and induced glioma cell motility. In contrast, S59D mutation resulted in the rapid degradation of P311 and reduced glioma cell migration.

Function : May have roles in neural function. Ectopic expression augments motility of gliomas. Promotes also axonal regeneration (By similarity). May also have functions in cellular differentiation (By similarity). Induces differentiation of fibroblast into myofibroblast and myofibroblast ameboid migration. Increases retinoic-acid regulation of lipid-droplet biogenesis (By similarity). Down-regulates the expression of TGFB1 and TGFB2 but not of TGFB3 (By similarity). May play a role in the regulation of alveolar generation.

Subunit : Interacts with the latency-associated peptides (LAP) of TGFB1 and TGFB2; the interaction results in a decrease in TGFB autoinduction (By similarity). Interacts with FLNA.

Subcellular Location : Cytoplasm.

Post-translational modifications : Phosphorylated on Ser-59. Phosphorylation decreases stability and activity.

Database links :

UniProtKB/Swiss-Prot: Q16612.1<br>

GI:2833275

P311 protein 還稱：PTZ17 (pentylenetetrazd117)又稱：相關(guān)蛋白。該蛋白與后增生修復(fù)有關(guān)。

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★神經(jīng)再生相關(guān)蛋白抗體規(guī)格 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測序、載體構(gòu)建等

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