p19ARF基因抗體品牌 英文名稱  Anti-p19ARF

中文名稱  p19ARF基因抗體品牌 

別    名 MTS1; ARF; CDK4 inhibitor P16 INK4; CDKN2A; Cyclin dependent kinase inhibitor 2A; INK4; P19; P19ARF; p19INK4a.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg 1ml/1mg

抗體來(lái)源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 信號(hào)轉(zhuǎn)導(dǎo) 細(xì)胞凋亡 細(xì)胞周期蛋白 轉(zhuǎn)錄調(diào)節(jié)因子

蛋白分子量  predicted molecular weight: 19kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human P19ARF

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

p19ARF基因抗體品牌 產(chǎn)品應(yīng)用   WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 P19ARF Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.

Function : Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development.

Subunit : Does not interact with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6. Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1/TOPOI and UBE2I/UBC9. Interacts with TBRG1 and COMMD1. Interacts with CDKN2AIP and E4F1.

Subcellular Location : Nucleus, nucleolus. Nucleus, nucleoplasm.

p19ARF是基因，通過(guò)干擾p53的功能，導(dǎo)致細(xì)胞周期調(diào)節(jié)紊亂和細(xì)胞凋亡障礙，使細(xì)胞發(fā)生，與多種的形成有關(guān)。

目前對(duì)p19ARF的研究雖然取得了很大的進(jìn)展，但是還存在一些亟待解決的問(wèn)題，如:p19ARF蛋白的表達(dá)調(diào)控還不是十分清楚;如何正確地認(rèn)識(shí)并理解p19ARF的作用機(jī)制，p19ARF對(duì)發(fā)生的研究有重要的理論意義;目前的許多證據(jù)大部分來(lái)源于動(dòng)物實(shí)驗(yàn)，p19ARF與人類的關(guān)系還需要進(jìn)一步研究。

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

p19ARF基因抗體品牌 全：公司提供上萬(wàn)種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

新：產(chǎn)品更新速度較快，基本上每周都有新產(chǎn)品出現(xiàn)。

優(yōu)：產(chǎn)品質(zhì)量好，投訴比較少。

好：我公司具有優(yōu)質(zhì)的技術(shù)團(tuán)隊(duì)，產(chǎn)品一旦售出，實(shí)驗(yàn)過(guò)程中遇到困難可提供在線技術(shù)咨詢。使您使用產(chǎn)品時(shí)沒(méi)有任何的后顧之憂。

正在熱銷(xiāo)的相關(guān)產(chǎn)品：

Anti-FSCN1/FITC 熒光素標(biāo)記纖維束蛋白同源物1/肌動(dòng)蛋白集束蛋白/圓線蟲(chóng) 抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Nm23-H2/FITC 熒光素標(biāo)記抑制基因抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Brs3/Bombesin Receptor 3 蛙皮素受體3抗體 規(guī)格 0.1ml

ARIP2a(Activin receptor 2A) 激活素受體2A/激活素受體相互作用蛋白2a抗原 0.5mg

HRAS+KRAS 英文名稱： 轉(zhuǎn)化蛋白p21抗體(原癌基因H-ras抗體) 0.2ml

Rhesus antibody Rh RIN1 RAS抑制蛋白1抗體 規(guī)格 0.2ml

Anti-Nm23-H2/FITC 熒光素標(biāo)記抑制基因抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-MRP2/FITC 熒光素標(biāo)記多藥耐藥相關(guān)蛋白2抗體Multi-class antibodies規(guī)格： 0.2ml

Anti-Phospho-Tuberin/TSC2 (Ser1387)/FITC 熒光素標(biāo)記磷酸化馬鈴薯球蛋白(性硬化)抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh 4EBP2 eIF4E結(jié)合蛋白2抗體 規(guī)格 0.1ml

Mouse Anti-rat IgG/PE-CY5 PE-CY5標(biāo)記的小鼠抗大鼠IgG 0.1ml

CKS2 英文名稱： 細(xì)胞周期蛋白依賴性激酶調(diào)節(jié)亞基2抗體 0.2ml

Rhesus antibody Rh PSME3/PA28 gamma 蛋白酶體激活因子PA28γ抗體 規(guī)格 0.2ml

Anti-Phospho-Tuberin/TSC2 (Ser1387)/FITC 熒光素標(biāo)記磷酸化馬鈴薯球蛋白(性硬化)抗體IgGMulti-class antibodies規(guī)格： 0.2ml

VC(Human Vitamin C) ELISA Kit 人維生素CMulti-class antibodies規(guī)格： 48T

Anti-PLG 纖維蛋白溶酶原抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Hexokinase II/HxK2 己糖激酶2抗體 規(guī)格 0.2ml

MYS ELISA Kit 大鼠肌球蛋白 96T

phospho-PKC Epsilon(Ser729) 英文名稱： 磷酸化蛋白激酶C抗體 PKC ε 0.1ml

C9orf30 英文名稱： 9號(hào)染色體開(kāi)放閱讀框30抗體 0.2ml

Anti-PLG 纖維蛋白溶酶原抗體Multi-class antibodies規(guī)格： 0.2ml

CL-0370J774A.1(小鼠單核巨噬細(xì)胞)5×106cells/瓶×2

ART4 Others Mouse 小鼠 ART4 / CD297 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

小鼠上皮細(xì)胞完全培養(yǎng)基 100mL

HOC 肝卵圓細(xì)胞 HOC of hepatic oval cells DMEM+10% FBS

IL1B Protein Canine 重組狗 IL-1 beta / IL1B 蛋白

NCI-H295R(人腎上腺皮質(zhì)腺癌細(xì)胞) 5×106cells/瓶×2 SK-N-SH(神經(jīng)母細(xì)胞瘤細(xì)胞)

CL-0367IM-9(人外周血B淋巴細(xì)胞)5×106cells/瓶×2

CD58 Others Human 人 LFA-3 / CD58 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

人平滑肌細(xì)胞總RNAHPSMC NA

MDA-MB-231細(xì)胞，人癌細(xì)胞 BALB/c小鼠ES細(xì)胞,B/c-ES細(xì)胞 BPH-1, 人細(xì)胞

NCI-H1975(人) 5×106cells/瓶×2

HDMEC adult Pellet 人真皮微內(nèi)皮細(xì)胞團(tuán)塊(成年捐獻(xiàn)者) > 1 mio.cells 小氣道上皮細(xì)胞培養(yǎng)基SAEpiCM-prf

p19ARF基因抗體品牌 人表皮黑色素細(xì)胞-中色素(HEM)( 5×105 )

人低轉(zhuǎn)移細(xì)胞株;PC-3M-2B4

小鼠骨髓間充質(zhì)干細(xì)胞(BMSCs)

人結(jié)直腸腺癌細(xì)胞;HCT 116 [HCT116] 大鼠腎足突細(xì)胞完全培養(yǎng)基 100mL

P19 小鼠

MMP9 Others Mouse 小鼠 MMP-9 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

技術(shù)外包服務(wù):

★p19ARF基因抗體品牌 分子生物學(xué)：質(zhì)粒抽提、PCR、Q-PCR、RT-PCR、分子生物學(xué)：基因合成、引物合成、基因測(cè)序、載體構(gòu)建等

★蛋白工程：原核、哺乳動(dòng)物蛋白表達(dá)系統(tǒng)等

★病毒包裝：腺病毒、慢病毒等

★抗體工程：磁珠分選、病理染色、WB、ELISA、IP、IF、IHC、FACS、Confocal等等

★細(xì)胞工程：細(xì)胞表型分析(凋亡、增殖、周期、遷移、侵襲、修復(fù)、克隆形成)、細(xì)胞培養(yǎng)、細(xì)胞膜制備、穩(wěn)定細(xì)胞株構(gòu)建、細(xì)胞RNAi技術(shù)等等。