抗體的生物素化標(biāo)記實驗要點：

1. 蛋白酶體PSMα1抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標(biāo)記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-Proteasome 20S C2 

中文名稱   蛋白酶體PSMα1抗體品牌 

別    名  20S Proteasome α1; 30 kDa prosomal protein; HC 2; HC2; Macropain subunit C2; Macropain subunit nu; Multicatalytic endopeptidase complex subunit C2; NU antibody PROS 30; PROS-30; PROS30; Proteasome (prosome macropain) subunit alpha type 1; Proteasome alpha 1 subunit; Proteasome component C2; Proteasome nu chain; Proteasome subunit alpha type 1; Proteasome subunit alpha type I; Proteasome subunit alpha type-1; Proteasome subunit nu; Protein P30 33K; PSA1_HUMAN; PSC 2; PSC2; PSMA 1; psmA1.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Cow, Horse 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細胞生物 免疫學(xué)

蛋白分子量  predicted molecular weight: 30kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human Proteasome 20S C2 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

蛋白酶體PSMα1抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Ubiquitin-dependent proteolysis mediates selective destruction of various cell cycle regulators, transcription factors and tumor suppressors. In eukaryotic cells, selective breakdown of cellular proteins is ensured by their ubiquitination and subsequent degradation by the 26S proteasome. At specific stages of development, embryo- and tissue-specific components of the 26S proteasome form, facilitating proteolysis. 20S Proteasome ?, also designated macropain subunit C2 or PROS-30, is a prosomal protein involved in a non-lysosomal ATP/ubiquitin-dependent proteolytic pathway. The entire proteasome is composed of at least 15 non-identical subunits which form a highly-ordered ring-shaped structure.

Function : The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Mediates the lipopolysaccharide-induced signal transduction in the macrophage proteasome (By similarity). Might be involved in the anti-inflammatory response of macrophages during the interaction with C.albicans heat-inactivated cells.

Subunit : The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with bacterial

Subcellular Location : Cytoplasm. Nucleus.

Database links : UniProtKB/Swiss-Prot: P25786.1

抗體的鑒定：

1）蛋白酶體PSMα1抗體品牌 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應(yīng),瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。

Anti-GDF8/MSTN/FITC 熒光素標(biāo)記羊抗生長分化因子8/肌肉抑制素抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-NSE/FITC 熒光素標(biāo)記神經(jīng)元特異性烯醇化酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh BMPR1A/CD292 骨成型蛋白受體1A抗體 規(guī)格 0.1ml

GHRF (GHRH) 生長激素釋放激素(因子)(抗原) 0.5mg

hnRNP U 英文名稱： 異質(zhì)核糖核蛋白U抗體 0.2ml

Rhesus antibody Rh RED/CSA2 軟相關(guān)蛋白2抗體 規(guī)格 0.2ml

Anti-NSE/FITC 熒光素標(biāo)記神經(jīng)元特異性烯醇化酶抗體IgGMulti-class antibodies規(guī)格： 0.2ml

POMC(Human pro-opiomelanocortin) ELISA Kit 人阿黑皮素原Multi-class antibodies規(guī)格： 48T

Anti-PKB/AKT-1 蛋白激酶B(小鼠來源抗體)Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh HIP2 泛素蛋白連接酶E2抗體 規(guī)格 0.2ml

ANG- I R(Human angiotension I receptor Antibody) ELISA Kit 人緊張素Ⅰ受體抗體 96T

EV71 polyprotein VP1 英文名稱： 腸道病毒71型/病毒抗體 0.2ml

CD34 英文名稱： CD34抗體 0.1ml

Anti-PKB/AKT-1 蛋白激酶B(小鼠來源抗體)Multi-class antibodies規(guī)格： 0.1ml

RF(Human rheumatoid factor) ELISA Kit 人類因子Multi-class antibodies規(guī)格： 48T

Anti-Phospho-SEK1/MKK4 (Thr261) 磷酸化原活化蛋白激酶激酶4抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh HSPBAP1 熱休克蛋白27相關(guān)蛋白1抗體 規(guī)格 0.2ml

T(Mouse Testoterone)ELISA Kit 小鼠睪酮 96T

phospho-PTPN6(Tyr564) 英文名稱： 磷酸化蛋白酪酸磷酸酶1C抗體 0.1ml

CCDC148 英文名稱： 卷曲螺旋結(jié)構(gòu)域蛋白148抗體 0.2ml

Anti-Phospho-SEK1/MKK4 (Thr261) 磷酸化原活化蛋白激酶激酶4抗體Multi-class antibodies規(guī)格： 0.1ml

CL-0467WPMY-1(人正?；|(zhì)永生化細胞)5×106cells/瓶×2

CXCL16 Others Rat 大鼠 CXCL16 / SR-PSOX 人細胞裂解液 (陽性對照)

大鼠虹膜色素上皮細胞完全培養(yǎng)基 100mL

U-373MG人 U-373MG glioma cells DMEM+10% FBS

IL1B Protein Rabbit 重組兔 IL-1 beta / IL1B 蛋白

NCI-H358(人非小細胞細胞) 5×106cells/瓶×2 PC-12(腎上腺嗜鉻細胞瘤細胞)

CL-0464USMC(大鼠平滑肌細胞)5×106cells/瓶×2

CD44 Others Human 人 CD44 人細胞裂解液 (陽性對照)

人肝內(nèi)膽管上皮細胞RNAHIBEpiC miRNA5 μg

5637(HTB-9)細胞，人細胞 大鼠細胞(癌細胞接種到*成瘤),Walker-256細胞 MLF, 小鼠淋巴成纖維細胞

HO-8910PM(人高轉(zhuǎn)移細胞) 5×106cells/瓶×2

hMSC-AT-c 人類脂肪組織間質(zhì)干細胞(hMSC-AT) 500,000cells 小鼠背脊髓神經(jīng)元MN-dsc

蛋白酶體PSMα1抗體品牌 PROCR Others Cynomolgus 食蟹猴 Epcr / PROCR 人細胞裂解液 (陽性對照)

羅猴胎腎細胞;MA104

CL-0229T84(人腺癌肺轉(zhuǎn)移細胞)5×106cells/瓶×2

SH-77細胞，小細胞 人T細胞細胞,T-CEM細胞 人T轉(zhuǎn)基因細胞;Jurkat D,E

A-375(人惡性細胞) 5×106cells/瓶×2

BACE1 Others Human 人 BACE1 / ASP2 人細胞裂解液 (陽性對照)