抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化蛋白酶體PSMα3抗體費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來(lái)篩選最適條件；

3.用NHSB量過(guò)量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴(lài)氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購(gòu)信息：
英文名稱(chēng)  Anti-Proteasome 20S alpha 3(Ser250) 

中文名稱(chēng)   磷酸化蛋白酶體PSMα3抗體費(fèi)用 

別    名  Proteasome 20S alpha 3 (phospho S250); 20S Proteasome α3; HC8; Macropain subunit C8; Multicatalytic endopeptidase complex subunit C8; Proteasome (prosome macropain) subunit alpha type 3; Proteasome alpha 3 subunit; Proteasome component C8; Proteasome subunit alpha type 3; Proteasome subunit alpha type-3; Proteasome subunit C8; PSA3_HUMAN; PSC3; PSMA3.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來(lái)源   Rabbit

克隆類(lèi)型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep 

產(chǎn)品類(lèi)型   一抗 磷酸化抗體  

研究領(lǐng)域    細(xì)胞生物 細(xì)胞周期蛋白 細(xì)胞分化

蛋白分子量  predicted molecular weight: 28kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human Proteasome 20S alpha 3 around the phosphorylation site of Ser250 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸化蛋白酶體PSMα3抗體費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Function : The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Binds to the C-terminus of CDKN1A and thereby mediates its degradation. Negatively regulates the membrane trafficking of the cell-surface thromboxane A2 receptor (TBXA2R) isoform 2.

Subunit : The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Interacts with AURKB. Interacts with CDKN1A. Interacts with -1 TAT protein. Interacts with hepatitis C virus (HCV) F protein. Interacts with Epstein-Barr virus EBNA3 proteins. Interacts with MDM2 and RB1. Interacts with the C-terminus of TBXA2R isoform 2.

Subcellular Location : Cytoplasm. Nucleus.

Database links : UniProtKB/Swiss-Prot: P25788.2

抗體的鑒定：

1）磷酸化蛋白酶體PSMα3抗體費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來(lái)鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來(lái)表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線(xiàn),計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無(wú)窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫(kù)侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

Anti-FAM3A/FITC 熒光素標(biāo)記*衍生因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-NDRG1 (Thr346)/FITC 熒光素標(biāo)記磷酸化分化相關(guān)基因NDRG1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh C12ORF54 12號(hào)染色體開(kāi)放閱讀框54抗體 規(guī)格 0.2ml

PDGF-A 血小板源性生長(zhǎng)因子-1(抗原) 0.5mg

hnRNP M 英文名稱(chēng)： 異質(zhì)性核糖核蛋白M抗體 0.2ml

Rhesus antibody Rh RNF32 環(huán)指蛋白32抗體 規(guī)格 0.2ml

Anti-Phospho-NDRG1 (Thr346)/FITC 熒光素標(biāo)記磷酸化分化相關(guān)基因NDRG1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-COX10/FITC 熒光素標(biāo)記COX10抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-PKB/FITC 熒光素標(biāo)記蛋白激酶B抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ASAP3 GTP酶激活蛋白ASAP3抗體 規(guī)格 0.2ml

Donkey Anti-rabbit IgG/Alexa Fluor 350 Alexa Fluor 350標(biāo)記的驢抗兔IgG 0.1ml

GJA3 英文名稱(chēng)： 間隙連結(jié)蛋白46/相關(guān)蛋白抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-Monkey IgM/Gold 膠體金標(biāo)記的兔抗猴IgM 規(guī)格 0.5ml

Anti-PKB/FITC 熒光素標(biāo)記蛋白激酶B抗體IgGMulti-class antibodies規(guī)格： 0.2ml

SK(Human Streptokinase) ELISA Kit 人鏈激酶Multi-class antibodies規(guī)格： 48T

Anti-NGF-beta 神經(jīng)生長(zhǎng)因子-β抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh IL-17B 白介素-17B抗體 規(guī)格 0.1ml

IL-2sR Beta ELISA Kit 大鼠白介素2可溶性受體β鏈 96T

phospho-PTPN7 (Ser93) 英文名稱(chēng)： 磷酸化非受體型蛋白酪酸磷酸酶7抗體 0.1ml

CD15 英文名稱(chēng)： 階段特異性胚胎表面抗原-1抗體 0.1ml

Anti-NGF-beta 神經(jīng)生長(zhǎng)因子-β抗體Multi-class antibodies規(guī)格： 0.1ml

CM-H004人肺大內(nèi)皮細(xì)胞完全培養(yǎng)基100mL

SUSD4 Others Human 人 SUSD4 / Sushi domain-coaining 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

原代軟骨細(xì)胞特制基礎(chǔ)培養(yǎng)基Many types of cells包裝：500/250/100ml

DU145細(xì)胞，人細(xì)胞 人癌細(xì)胞,LCC1細(xì)胞 間充質(zhì)干細(xì)胞培養(yǎng)基MSCM-acf-prf

婆羅門(mén)牛皮膚成纖維樣細(xì)胞;BOS-4

IL2RG Others Mouse 小鼠 IL2RG 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

CM-H003人肺大動(dòng)脈平滑肌細(xì)胞完全培養(yǎng)基100mL

NGFR Others Human 人 NGFR/ P75 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

I型膠原酶(含10mL酶解緩沖液) 1mL

CAL 27-EGFP(CMV-EGFP慢病毒構(gòu)建穩(wěn)定株)人舌鱗癌細(xì)胞 CAL 27-EGFP (CMV-EGFP leiviral consuct stable sain) of human tongue squamous cell carcinoma DMEM+10% FBS

IL21 Protein Mouse 重組小鼠 IL21 / Ierleukin 21 蛋白

人表皮黑色素細(xì)胞-深色素(HEM)( 5×105 ) MN-h, 小鼠海馬神經(jīng)元 Mouse

磷酸化蛋白酶體PSMα3抗體費(fèi)用 CD53 Others Cynomolgus 食蟹猴 CD53 (aa 107-181) 人細(xì)胞裂解液 (陽(yáng)性對(duì)照)

子宮內(nèi)膜上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

豚鼠胚胎細(xì)胞;104C1

LTEP-a-2細(xì)胞，人 猴腎細(xì)胞系,VERO E6細(xì)胞 CM-M050小鼠卵巢顆粒細(xì)胞完全培養(yǎng)基100mL

SHG-44 (人細(xì)胞) 5×106cells/瓶×2

NHEK.f-c pooled 正常人表皮角質(zhì)形成細(xì)胞(NHEK)少年，混合 500,000cells 原代上皮細(xì)胞特制無(wú)血清添加劑Many types of cells包裝：1ml