產(chǎn)品訂購信息：
英文名稱  Anti-PMS1 

中文名稱   錯(cuò)配修復(fù)基因PMS1抗體品牌 

別    名  DNA mismatch repair protein PMS1; HNPCC3; hPMS1; Human homolog of yeast mutL; Mismatch repair gene PMSL1; pms1; PMS1 postmeiotic segregation increased 1 (S. cerevisiae); PMS1 postmeiotic segregation increased 1; PMS1 protein homolog 1; PMS1_HUMAN; PMSL1; Rhabdomyosarcoma antigen MU RMS 40.10B; Rhabdomyosarcoma antigen MU RMS 40.10E.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Cow, Horse, Sheep 

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 106kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PMS1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

錯(cuò)配修復(fù)基因PMS1抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:50-200

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression. Two additional homologs of the prokaryotic MutL gene, designated PMS1 and PMS2, have been identified and shown to be mutated in the germline of HNPCC patients.

Function : Probably involved in the repair of mismatches in DNA.

Subunit : The MutL-beta complex is a heterodimer of PMS1 and MLH1.

Subcellular Location : Nucleus.

Similarity : Belongs to the DNA mismatch repair MutL/HexB family.

Contains 1 HMG box DNA-binding domain.

Database links : UniProtKB/Swiss-Prot: P54277.1

Anti-E.coli O139/Biotin 生物素標(biāo)記抗志賀毒素大腸桿菌O139抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Metal ion transporter/FITC 熒光素標(biāo)記擬南介金屬離子轉(zhuǎn)運(yùn)蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh C6orf58 6號(hào)染色體開放閱讀框58抗體 規(guī)格 0.2ml

E-Selectin E選擇素抗原 0.5mg

HN1 英文名稱： 雄激素調(diào)節(jié)蛋白2抗體 0.1ml

Rhesus antibody Rh SCNN1D/δENaC 上皮離子通道蛋白D抗體 規(guī)格 0.2ml

Anti-Metal ion transporter/FITC 熒光素標(biāo)記擬南介金屬離子轉(zhuǎn)運(yùn)蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-CNTF/FITC 熒光素標(biāo)記睫狀神經(jīng)營養(yǎng)因子抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-p-Klf5/CKLF /FITC 熒光素標(biāo)記磷酸化腸道內(nèi)富含的Kruppel樣因子5抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh CCDC54/NYD-SP17 卷曲螺旋結(jié)構(gòu)域蛋白54抗體 規(guī)格 0.2ml

Histone H1b(Histone H1.4) 組蛋白H1b抗原 0.5mg

Histone H3 (Tri Methyl K27) 英文名稱： 基化組蛋白H3抗體 0.1ml

Rhesus antibody Rh SNAI2/SLUG 鋅指轉(zhuǎn)錄因子Slug抗體 規(guī)格 0.1ml

Anti-p-Klf5/CKLF /FITC 熒光素標(biāo)記磷酸化腸道內(nèi)富含的Kruppel樣因子5抗體IgGMulti-class antibodies規(guī)格： 0.2ml

IFA(Human intrinsic factor antibody) ELISA Kit 人抗內(nèi)因子抗體Multi-class antibodies規(guī)格： 48T

Anti-IL-20R alpha/IL20RA 白介素20受體α鏈抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh L-Selectin/CD62L L選擇素抗體 規(guī)格 0.1ml

GLP-1(human) 人胰素樣肽1 96T

phospho-RAD51(Thr309) 英文名稱： 磷酸化Rad51抗體 0.1ml

phospho-Cyclin E (Thr395) 英文名稱： 磷酸化周期素E抗體 0.1ml

Anti-IL-20R alpha/IL20RA 白介素20受體α鏈抗體Multi-class antibodies規(guī)格： 0.2ml

CM-H020人胰島β細(xì)胞完全培養(yǎng)基100mL

STATH Others Human 人 Statherin / STATH 人細(xì)胞裂解液 (陽性對(duì)照)

真皮淋巴上皮細(xì)胞Many types of cells包裝：5 × 105次方(1ml)

293sars181A細(xì)胞，Sars蛋白表達(dá)株 人細(xì)胞,HBL-100細(xì)胞 腎系膜細(xì)胞培養(yǎng)基MCM-prf

大額牛皮膚成纖維樣細(xì)胞;BOS-2

IL2RB Others Human 人 IL2Rβ 人細(xì)胞裂解液 (陽性對(duì)照)

CM-H020人胰島β細(xì)胞完全培養(yǎng)基100mL

HA Others H5N1 甲型 H5N1 (A/Cambodia/S1211394/2008) 血凝素HA1 (Hemagglutinin) 人細(xì)胞裂解液 (陽性對(duì)照)

胰蛋白酶(含10mL 酶解緩沖液) 1mL

U-373MG-EGFP-puro[U-373MG-pLVT7](慢病毒構(gòu)建穩(wěn)定株)人 U-373MG-EGFP-puro[U-373MG-pLVT7] (leiviral consuct stable sain) of human brain glioma cells 1640+10%Hyclone 滅活血清+2μg/ml puromycin

IL21 Protein Rat 重組大鼠 IL21 / Ierleukin 21 蛋白 (His 標(biāo)簽)

人表皮角質(zhì)細(xì)胞-新生兒(HEK-n)( 5×105 ) 細(xì)胞名稱 種屬

錯(cuò)配修復(fù)基因PMS1抗體品牌 CD59 Others Rat 大鼠 CD59 / CD59A / MAC / IP 人細(xì)胞裂解液 (陽性對(duì)照)

EB病毒轉(zhuǎn)化的人B淋巴細(xì)胞;KM9803

人子宮內(nèi)膜上皮細(xì)胞完全培養(yǎng)基 100mL

FO細(xì)胞，小鼠細(xì)胞 3T6-Swiss albino(胚胎成纖維細(xì)胞) 人臍內(nèi)皮細(xì)胞(人細(xì)胞污染);ECV-304 [ECV304;ECV 304]

CXCL9 Protein Mouse 重組小鼠 CXCL9 / MIG / C-X-C motif chemokine 9 蛋白

S-180(小鼠細(xì)胞) 5×106cells/瓶×2 人 Niemann-Pick disease type C2 / NPC2 人細(xì)胞裂解液 (陽性對(duì)照)

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 錯(cuò)配修復(fù)基因PMS1抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競(jìng)爭(zhēng)作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）錯(cuò)配修復(fù)基因PMS1抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競(jìng)爭(zhēng)抑制試驗(yàn)測(cè)定。以不同濃度抗原和近似抗原分別做競(jìng)爭(zhēng)抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測(cè)定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。