產(chǎn)品訂購信息：
英文名稱  Anti-PARP (N-Terminus) 

中文名稱   多腺苷二磷酸多聚酶抗體(N端)說明書 

別    名  ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應   Human, Mouse, Rat, Dog, Cow 

產(chǎn)品類型   一抗  

研究領域    染色質(zhì)和核信號 細胞凋亡

蛋白分子量  predicted molecular weight: 111kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PARP 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

多腺苷二磷酸多聚酶抗體(N端)說明書 產(chǎn)品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008].

Function : Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production.

Subunit : Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK.

Subcellular Location : Nucleus.

Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites.

S-nitrosylated, leading to inhibit transcription regulation activity.

Similarity : Contains 1 BRCT domain.

Contains 1 PARP alpha-helical domain.

Contains 1 PARP catalytic domain.

Contains 2 PARP-type zinc fingers.

Database links : UniProtKB/Swiss-Prot: P09874.4

PARP(poly ADP-ribose polymerase)是DNA修復酶。

PARP是細胞凋亡核心成員半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA損傷修復與細胞凋亡中發(fā)揮著重要作用。Anti-PARP p85 是特意的PARPp85片段的特異抗體，由caspase剪切116kDa完整分子而得到的。

PARP是存在于多數(shù)真核細胞中的一個多功能蛋白質(zhì)翻譯后修飾酶。它通過識別結(jié)構(gòu)損傷的DNA片段而被激活，對聚腺苷二磷酸核糖聚合酶PARP被認為是DNA損傷的感受器。它還能對許多核蛋白進行聚腺苷二磷酸核糖基化。因此，在DNA損傷修復與細胞凋亡中發(fā)揮著重要作用，端錨聚合酶在癌細胞端粒結(jié)構(gòu)的調(diào)控機制中有重要作用。

CA(Human Cholic acid) ELISA Kit 人膽酸Multi-class antibodies規(guī)格： 48T

Anti-Phospho-HSP27 (Ser15) 磷酸化熱休克蛋白27抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh MARK4 絲氨酸/蘇氨酸蛋白激酶MARK4抗體 規(guī)格 0.1ml

PPIL1(Human peptidylprolyl isomerase(cyclophilin)-like 1) ELISA Kit 人肽酰脯酰異構(gòu)酶(親環(huán)蛋白)樣1 96T

RSBN1L 英文名稱： 細胞堿性蛋白1樣蛋白抗體 0.1ml

Cecropin 英文名稱： 殺菌肽/天蠶抗菌肽單克隆抗體 1ml

Anti-Phospho-HSP27 (Ser15) 磷酸化熱休克蛋白27抗體Multi-class antibodies規(guī)格： 0.1ml

M2-PK(Mouse Pyruvate Kinase) ELISA Kit 小鼠酸激酶M2型同工酶Multi-class antibodies規(guī)格： 48T

Anti-CD58/LFA-3/FITC 熒光素標記淋巴細胞功能相關抗原-3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh DPY19L1 短粗矮胖19蛋白樣1抗體 規(guī)格 0.2ml

RV IgM 胞病毒 IgM(捕獲法二步法)

Midkine 英文名稱： 中期因子/肝素結(jié)合生長因子抗體 0.1ml

Annexin A7 英文名稱： 膜粘連蛋白7抗體 0.1ml

Anti-CD58/LFA-3/FITC 熒光素標記淋巴細胞功能相關抗原-3抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-Lyn (Tyr507) /FITC 熒光素標記膜相關蛋白酪氨酸激酶Lyn抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-phospho-Tau protein (Ser422)/FITC 熒光素標記磷酸化微管相關蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ADCK3/CABC1 伴侶蛋白bc1同源復合體抗體 規(guī)格 0.2ml

Rabbit Anti-dog IgG/FITC FITC標記的兔抗狗IgG 0.3ml

GBP5 英文名稱： G蛋白結(jié)合蛋白5抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-chicken IgG/Cy7 Cy7標記的兔抗雞IgG 規(guī)格 0.1ml

Anti-phospho-Tau protein (Ser422)/FITC 熒光素標記磷酸化微管相關蛋白抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠基質(zhì)金屬蛋白酶7(MMP-7)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人鞘脂激活蛋白原(PSAP)免疫試劑盒 human prosaposin,PSAP Elisa kit

Mousemaixmetalloproteinase2/GelatinaseA,MMP-2ELISAKit小鼠基質(zhì)金屬蛋白酶2/明膠酶A(MMP-2/GelatinaseA)ELISA試劑盒規(guī)格：96T/48T

P27蛋白表達西方雜交分析試劑盒5次

Humanoctameranscriptionfactor2B,OTF2BELISA試劑盒人八聚體轉(zhuǎn)錄因子(OTF2B)ELISA試劑盒規(guī)格：96T/48T

humaeceptoNFRSF-ieractingserine-threoninekinase2,RIPK2ELISAKit人受體TNFRSF結(jié)合絲氨酸蘇氨酸激酶2(RIPK2)ELISA試劑盒規(guī)格：96T/48T

大鼠β淀粉樣蛋白1-40(Aβ1-40)ELISA試劑盒 ，英文名： Aβ1-40 ELISA Kit

Mouse coicoopin releasing hormone (CRH) ELISA Kit 小鼠促腎上皮質(zhì)激素釋放激素(CRH)ELISA試劑盒

ELISA 小鼠抑瘤素mouse Oncostatin M(mouse OSM) 48T/96T 進口分裝

CLIAKitforIL-1ELISAKit大鼠白介素1規(guī)格：48T/96T

通用型南瓜黃化花葉病毒(ZucchiniYellowMosaicVirus;ZYMV)試劑盒20次

ELISAKitaGT大鼠緊張素原規(guī)格：48T/96T

多腺苷二磷酸多聚酶抗體(N端)說明書 大鼠白介素8(IL-8/CXCL8)ELISA試劑盒 ，英文名： IL-8/CXCL8 ELISA Kit

Mouse ierleukin 13 (IL-13) ELISA Kit 小鼠白介素13(IL-13)ELISA試劑盒

ELISA 小鼠神經(jīng)膠質(zhì)細胞衍化營養(yǎng)因子(mouse GDNF) 48T/96T 進口分裝

CLIAKitfori-PTHELISAKit大鼠全段甲狀旁腺素規(guī)格：48T/96T

通用型非洲(Africanswinefevervirus;ACSF)試劑盒20次

ELISAKitLPL大鼠脂蛋白脂酶規(guī)格：48T/96T

抗體的生物素化標記實驗要點：

1. 多腺苷二磷酸多聚酶抗體(N端)說明書 如在反應混合液中有疊氮鈉或游離氨基存在,會抑制標記反應。因此,蛋白質(zhì)在反應前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當則影響標記的效率,應先用幾個不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點可能因此被封閉,導致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時可加入去污劑如 Triton x-100, Tween20等；

5.當游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點時,或位于酶的催化位點時,生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團,如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價結(jié)合；

7.交聯(lián)反應后,應充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細胞的熒光標記實驗中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細胞可導致高本底。

抗體的鑒定：

1）多腺苷二磷酸多聚酶抗體(N端)說明書 抗體的效價鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價。不同的抗原制備的抗體,要求的效價不一。鑒定效價的方法很多,包括有試管凝集反應,瓊脂擴散試驗,酶聯(lián)免疫吸附試驗等。常用的抗原所制備的抗體一般都有約成的鑒定效價的方法,以資比較。如制備抗抗體的效價,一般就采用瓊脂擴散試驗來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強。衡量特異性通常以交叉反應率來表示。交叉反應率可用競爭抑制試驗測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計算各自的結(jié)合率,求出各自在IC50時的濃度,并按公式計算交叉反應率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時,表示這一抗血清與其他抗原物質(zhì)的交叉反應率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol。抗體親和力的測定對抗體的篩選,確定抗體的用途,驗證抗體的均一性等均有重要意義。