抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酯酶Cβ3費(fèi)用 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對(duì) 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對(duì)生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對(duì)某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-PLCB3/PLC beta3 

中文名稱   磷酯酶Cβ3費(fèi)用 

別    名  PLC beta3; 1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3; 1-phosphatidylinositol-4; 5-bisphosphate phosphodiesterase beta-3; Phosphoinositide phospholipase C; Phosphoinositide phospholipase C-beta-3; Phospholipase C beta 3; Phospholipase C beta 3 (phosphatidylinositol specific); Phospholipase C-beta-3; PLC beta 3; PLC-beta-3; Plcb3; PLCB3_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg 0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Horse, Rabbit, Guinea Pig 

產(chǎn)品類型   一抗  

研究領(lǐng)域     信號(hào)轉(zhuǎn)導(dǎo) 激酶和磷酸酶

蛋白分子量  predicted molecular weight: 136kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PLC beta3 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酯酶Cβ3費(fèi)用 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Function : The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of receptor-mediated tyrosine kinase activators. For example, when activated by SRC, the encoded protein causes the Ras guanine nucleotide exchange factor RasGRP1 to translocate to the Golgi, where it activates Ras. Also, this protein has been shown to be a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. Two transcript variants encoding different isoforms have been found for this gene.

Subunit : Interacts with SHANK2. Interacts with LPAR2.

Subcellular Location : Membrane; Peripheral membrane protein.

Contains 1 PI-PLC X-box domain.

Contains 1 PI-PLC Y-box domain.

抗體的鑒定：

1）磷酯酶Cβ3費(fèi)用 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對(duì)抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

AT2R-Ab(Human Angiotensin II Receptor 2 antibody) ELISA Kit 人緊張素Ⅱ受體2抗體Multi-class antibodies規(guī)格： 48T

Anti-IFNGR1/CD119 干擾素-gamma受體1抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh MAOA 單氨氧化酶A抗體 規(guī)格 0.1ml

MASP(Human mannan binding lectin associated serine protease) ELISA Kit 人甘露聚糖結(jié)合凝集素相關(guān)絲酸蛋白酶 96T

RPE65 英文名稱： 視網(wǎng)膜色素上皮細(xì)胞特異性蛋白65抗體 0.2ml

Phospho-Beta Catenin (Ser45) 英文名稱： 磷酸化β 連環(huán)素蛋白抗體 0.1ml

Anti-IFNGR1/CD119 干擾素-gamma受體1抗體Multi-class antibodies規(guī)格： 0.2ml

P-Selectin/CD62P(Mouse P-Selectin) ELISA kit 小鼠P選擇素Multi-class antibodies規(guī)格： 48T

Anti-CD7/FITC 熒光素標(biāo)記抗CD7抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh E3 ubiquitin ligase/RNF218 E3泛素連接酶抗體 規(guī)格 0.2ml

MU IgG 病毒 IgG(間接法二步法)

Mitofusin 2 英文名稱： 線粒體融合蛋白Mfn2抗體 0.1ml

A Raf 英文名稱： A-Raf抗體 0.1ml

Anti-CD7/FITC 熒光素標(biāo)記抗CD7抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-ICAD/FITC 熒光素標(biāo)記Caspase 激活的脫氧核糖核酸酶抑制劑抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-Phospho-PRAS40 (Thr246)/FITC 熒光素標(biāo)記磷酸化蛋白激酶AKT底物1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ARTS1 脂肪細(xì)胞源性亮氨酸氨基肽酶抗體(內(nèi)皮細(xì)胞生長因子誘導(dǎo)蛋白) 規(guī)格 0.2ml

Donkey Anti-rabbit IgG/Cy7 Cy7標(biāo)記的驢抗兔IgG 0.1ml

Gcn1l1 英文名稱： 廣泛控制基酸合成1樣蛋白1抗體 0.2ml

Rhesus antibody Rh Rabbit anti-Monkey IgM whole serum 兔抗猴IgM抗血清 規(guī)格 1ml

Anti-Phospho-PRAS40 (Thr246)/FITC 熒光素標(biāo)記磷酸化蛋白激酶AKT底物1抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠甲狀旁腺激素(PTH) ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人去唾液酸糖蛋白受體(ASGPR)免疫試劑盒 Human asialoglyco protein receptor,ASGPR ELISA Kit

Mouselymphocytefunctionassociatedaigen3,LFA-3ELISAKit小鼠淋巴細(xì)胞功能相關(guān)抗原3(LFA-3/CD58)ELISA試劑盒規(guī)格：96T/48T

Insuli-alpha(抗人;抗兔;抗小鼠;抗大鼠)100微克

HumanN-MIDOsteocalcin,N-MID-OTELISA試劑盒人N端中段骨鈣素(N-MID-OT)ELISA試劑盒規(guī)格：96T/48T

Humanpunjecellaoaibody,PCA-1/YoELISAKit人抗浦肯野細(xì)胞抗體/抗Yo抗體(PCA-1/Yo)ELISA試劑盒規(guī)格：96T/48T

大鼠β位淀粉樣前體蛋白裂解酶2(BACE2)ELISA試劑盒 ，英文名： BACE2 ELISA Kit

Rabbit soluble adhesion molecule (Sam) ELISA Kit 兔子可溶性粘附分子(Sam)ELISA試劑盒

ELISA 小鼠硝基酪氨酸(mouse Niotyrosine) 48T/96T 進(jìn)口分裝

CLIAKitforIL-13(HumanIerleukin13)ELISAKIT人白介素13規(guī)格：48T/96T

通用型馬鈴薯X病毒(PotatoVirusX;PVX)試劑盒20次

ELISAKitANG-4大鼠生成素4規(guī)格：48T/96T

磷酯酶Cβ3費(fèi)用 大鼠白細(xì)胞介素1α(IL-1α)ELISA試劑盒 ，英文名： IL-1α ELISA Kit

Lens culinaris agglinin (LCA) ELISA Kit 扁豆凝集素(LCA)ELISA試劑盒

Mousegrowthhormonereleasingpeptide-Ghrelin,GHRP-GhrelinELISAKit 小鼠生長激素釋放肽ghrelin(GHRP-Ghrelin)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforHumanNon-PhosphorylatedInsulin-likegrowthfactorbindingprotein1ELISAKit人未磷酸化類生長因子結(jié)合蛋白-1規(guī)格：48T/96T

同位素真菌/酵母細(xì)胞蛋白核酸結(jié)合凝膠遷移電泳分析試劑盒20次

ELISAKitsICAM-1大鼠可溶性細(xì)胞間粘附分子1規(guī)格：48T/96T