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p400蛋白抗體說明書 英文名稱  Anti-p400

中文名稱  p400蛋白抗體說明書 

別    名 CAG repeat protein 32; CAGH32; DKFZP434I225; Domino homolog; E1A binding protein p400; EP 400; EP400; FLJ42018; FLJ45115; hDomino; KIAA1498; KIAA1818; p400 kDa SWI2/SNF2 related protein; P400 SWI2/SNF2-related protein; TNRC12; Trinucleotide repeat containing 12; Trinucleotide repeat containing gene 12 protein.

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse

產(chǎn)品類型   一抗  

研究領(lǐng)域     細(xì)胞生物 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 表觀遺傳學(xué)

蛋白分子量  predicted molecular weight: 344kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from human p400

亞    型  IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

p400蛋白抗體說明書 產(chǎn)品應(yīng)用   ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 NuAR is a multi-protein histone acetyltransferase complex that functions to acetylate the nucleosomal Histones H4 and H2A, thereby activating transcription of select target genes. p400, also known as EP400 (E1A binding protein p400) or mDomino, localizes to the nucleus and is a component of the NuAR complex. Expressed in brain, liver, thymus, lung, spleen, colon and kidney, p400 regulates the transcriptional activity of proteins such as MZF-1 and contributes to the ATPase and helicase activities of NuA4. p400 is a SWI2/ SNF2-related protein that can interact with the adenovirus oncoprotein E1A, thus activating the proapoptotic activity of E1A. The ability of p400 to regulate transcriptional and apoptotic activity suggests that the NuAR complex may be a crucial component of cell proliferation, transformation and, possibly, carcinogenesis. Five isoforms of p400 exist due to alternative splicing events.

Subunit : Component of the NuA4 histone acetyltransferase complex which contains the catalytic subunit KAT5/TIP60 and the subunits EP400, TRRAP/PAF400, BRD8/SMAP, EPC1, DMAP1/DNMAP1, RUVBL1/TIP49, RUVBL2, ING3, actin, ACTL6A/BAF53A, MORF4L1/MRG15, MORF4L2/MRGX, MRGBP, YEATS4/GAS41, VPS72/YL1 and MEAF6. May also participate in the formation of NuA4 related complexes which lack the KAT5/TIP60 catalytic subunit, but which include the SWI/SNF related protein SRCAP. The NuA4 complex interacts with MYC and the adenovirus E1A protein. EP400 interacts with TRRAP, RUVBL1 and RUVBL2. Interacts with ZNF42. Interacts with PHF5A (By similarity).

Subcellular Location : Nucleus.

Tissue Specificity : Ubiquitously expressed.

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