產(chǎn)品訂購信息：
英文名稱  Anti-PLEKHM1 

中文名稱   石骨癥相關(guān)蛋白PLEKHM1抗體品牌 

別    名  162 kDa adapter protein; AP162; PH domain-containing family M member 1; PKHM1_HUMAN; Pleckstrin homology domain-containing family M member 1; PLEKHM1.

濃    度   1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Pig, Horse, Rabbit 

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué)

蛋白分子量  predicted molecular weight: 117kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated synthetic peptide derived from human PLEKHM1 

亞    型   IgG

純化方法   affinity purified by Protein A

儲 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

石骨癥相關(guān)蛋白PLEKHM1抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 Involved in vesicular transport in the osteoclast (By similarity). May have a role in sialyl-lex-mediated transduction of apoptotic signals.

Tissue specificity: Expressed in placenta, liver, prostate, thymus, spleen, ovary, colon, colon carcinoma and peripheral blood lymphocytes (PBL). Weakly expressed in brain, lung, kidney, and testis. No expression in heart, skeletal muscle, pancreas and small intestine. Predominantly expressed in the breast carcinoma cell line MCF-7.

Involvement in disease:Defects in PLEKHM1 are the cause of osteopetrosis autosomal recessive type 6 (OPTB6); also known as autosomal recessive osteopetrosis intermediate form. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. Autosomal recessive osteopetrosis is usually associated with normal or elevated amount of non-functional osteoclasts.

Function : Involved in vesicular transport in the osteoclast (Bysimilarity). May have a role in sialyl-lex-mediated transduction ofapoptotic signals.

Subcellular Location : Cytoplasm (Potential).

Tissue Specificity : Expressed in placenta, liver, prostate,thymus, spleen, ovary, colon, colon carcinoma and peripheral bloodlymphocytes (PBL). Weakly expressed in brain, lung, kidney, andtestis. No expression in heart, skeletal muscle, pancreas and smallintestine. Predominantly expressed in the breast carcinoma cellline MCF-7.

DISEASE : Defects in PLEKHM1 are the cause of osteopetrosisautosomal recessive type 6 (OPTB6) [MIM:611497]; also known asautosomal recessive osteopetrosis intermediate form. Osteopetrosisis a rare genetic disease characterized by abnormally dense bone,due to defective resorption of immature bone. The disorder occursin two forms: a severe autosomal recessive form occurring in utero,infancy, or childhood, and a benign autosomal dominant formoccurring in adolescence or adulthood. Autosomal recessiveosteopetrosis is usually associated with normal or elevated amountof non-functional osteoclasts.

Similarity : Contains 2 PH domains.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 RUN domain.

Database links : UniProtKB/Swiss-Prot: Q9Y4G2.3

CYFRA21-1(Human cytokeratin fragment antigen 21-1) ELISA Kit 人細(xì)胞角蛋白21-1片段Multi-class antibodies規(guī)格： 48T

Anti-Phospho-HER3 (Tyr1222) 磷酸化HER3受體抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh MIA2 抑制活性蛋白2抗體 規(guī)格 0.2ml

uPA/PLAU(Human urokinase-type plasminogen activator) ELISA Kit 人尿激酶型纖溶酶原激活物 96T

RNF149 英文名稱： 環(huán)指蛋白149抗體 0.2ml

CEP72 英文名稱： 中心體蛋白72抗體 0.1ml

Anti-Phospho-HER3 (Tyr1222) 磷酸化HER3受體抗體Multi-class antibodies規(guī)格： 0.1ml

MPO(Mouse myeloperoxidase) ELISA Kit 小鼠髓過氧化物酶Multi-class antibodies規(guī)格： 48T

Anti-CEBP- Alpha /CLEBP Alpha /FITC 熒光素標(biāo)記轉(zhuǎn)錄調(diào)節(jié)因子C/EBP α 抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh Donkey Anti-Goat IgG/FITC FITC標(biāo)記的驢抗羊IgG 規(guī)格 0.3ml

IFN- Gamma ELISA Kit(mouse) 小鼠γ干擾素 96T

phospho-LRP5(Thr1492) 英文名稱： 磷酸化受體相關(guān)蛋白5抗體 0.1ml

Phospho-ATG4C(Ser398) 英文名稱： 磷酸化自噬相關(guān)蛋白4C抗體 0.1ml

Anti-CEBP- Alpha /CLEBP Alpha /FITC 熒光素標(biāo)記轉(zhuǎn)錄調(diào)節(jié)因子C/EBP α 抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-HDAC10/FITC 熒光素標(biāo)記組蛋白去乙?；?0抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-PEG10 /FITC 熒光素標(biāo)記高表達(dá)基因抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh BAALC 腦和急性胞漿蛋白抗體 規(guī)格 0.2ml

Actin Alpha /Alpha-SMA (Actin alpha , smooth muscle aorta) 肌動蛋白(抗原) 0.5mg

GGPS1 英文名稱： 法尼基二磷酸合酶1抗體 0.2ml

Rhesus antibody Rh Rabbit Anti-pig IgG/Cy3 Cy3標(biāo)記的兔抗豬IgG 規(guī)格 0.1ml

Anti-PEG10 /FITC 熒光素標(biāo)記高表達(dá)基因抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠巨噬細(xì)胞炎性蛋白3β(MIP-3β/ELC/CCL19)ELISA試劑盒 ，英文名： MIP-3β/ELC/CCL19 ELISA Kit

人白介素6(IL-6)ELISA檢測試劑盒HumanIerleukin6,IL-6ELISAKIT 96T/48T

高致病性豬藍(lán)耳病病毒(PRRSV-M)核酸檢測試劑盒(-PCR法) 48T

CLIAKitforNKBELISAKit(Ret)大鼠神經(jīng)肽B規(guī)格：48T/96T

體液3-磷酸甘油脫氫酶(GAPDH)活性酶動力比色法定量檢測試劑盒20次

ELISAKitTCR人T細(xì)胞受體規(guī)格：48T/96T

大鼠白介素1β(IL-1B)ELISA試劑盒 ，英文名： IL-1B ELISA Kit

Hamster receptor activator of nuclear factor kappa B ligand (RANKL) ELISA Kit 倉鼠核因子κB受體活化因子配基(RANKL)ELISA試劑盒

MouseypsinELISAKit 小鼠胰蛋白酶(ypsin)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforHumanN-Acetyl-Ser-Asp-Lys-Pro,AcSDKPELISAKit人N-乙?；?絲氨酰-天門冬酰-賴氨酰-脯氨酸規(guī)格：48T/96T

銅離子膜通道轉(zhuǎn)運(yùn)功能檢測試劑盒20次

ELISAKitsCD40L大鼠可溶性白細(xì)胞分化抗原40配體規(guī)格：48T/96T

石骨癥相關(guān)蛋白PLEKHM1抗體品牌 大鼠層連蛋白/板層素(LN)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人免疫球蛋白E Fc段受體Ⅱ(FcεRⅡ/CD23)免疫試劑盒 Human Receptor Ⅱ for the Fc region of immunoglobulin E,FcεRⅡ ELISA Kit

MouseVascularEndothelialcellGrowthFactor,VEGFELISAKit小鼠內(nèi)皮細(xì)胞生長因子(VEGF)ELISA試劑盒規(guī)格：96T/48T

石蠟切片組織CASPASE-2蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測試劑盒10/20次

HumanProstaglandinE2,PG-E2ELISA試劑盒人素E2(PGE2)ELISA試劑盒規(guī)格：96T/48T

Humahymopein,TP-5ELISAKit人胸腺五肽(TP-5)ELISA試劑盒規(guī)格：96T/48T

抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 石骨癥相關(guān)蛋白PLEKHM1抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

抗體的鑒定：

1）石骨癥相關(guān)蛋白PLEKHM1抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識別能力?？贵w的特異性高,它的識別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。