豬圓環(huán)Ⅱ型衣殼蛋白費(fèi)用 英文名稱  Anti-capsid protein

中文名稱  豬圓環(huán)Ⅱ型衣殼蛋白費(fèi)用 

別    名 ORF1; ORF2 (PCV-2; PCV 2; Porcine circovirus2; Porcine circovirus-2.)

產(chǎn)品屬性：

濃    度  1mg/1ml

規(guī) 格  0.2ml/200μg

抗體來源  Rabbit

克隆類型   polyclonal

交叉反應(yīng)   PCV2

產(chǎn)品類型   一抗  

研究領(lǐng)域    細(xì)胞生物 免疫學(xué) 細(xì)菌及

蛋白分子量  predicted molecular weight: 26kDa 

性    狀   Lyophilized or Liquid

免 疫 原  KLH conjugated synthetic peptide derived from PCV2 capsid protein

亞    型  IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

豬圓環(huán)Ⅱ型衣殼蛋白費(fèi)用 產(chǎn)品應(yīng)用   ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 There has been much uncertainty in the relationship between PCV2 and PMWS but Bolin et al demonstrated that Koch's postulates could be fulfilled by inoculating cesarean-derived, colostrum-deprived (CDCD) pigs with PCV2 and producing clinical signs of PMWS. Similar work has been performed using an infectious genomic clone of PCV2 in specific pathogen free (SPF) pigs to produce the gross and microscopic lymphoid lesions of PMWS. Although PCV2 has been shown to induce PMWS lesions in CDCD and SPF pigs, the presence of additional factors are required.

Function : Self-assembles to form the virion icosahedral capsid with a T=1 symmetry. This very small capsid (17 - 22 nm in diameter) allows the virus to be very stable in the environment and resistant to some disinfectants, including detergents. Essential for the initial attachment to heparan sulfate moities and chondroitin sulfate B of the host cell surface proteoglycans. After attachment, the virus is internalized in a clathrin-, caveolae- and dynamin-independent, actin and Rho-GTPase-mediated pathway and traffics to the nucleus. The capsid protein binds and transports the viral genome and Rep across the nuclear envelope.

Subunit : Homomultimer. Assembles in the nucleus, presumably in an immature form, then migrates to the cytoplasm once assembled as mature virion (Probable). Interacts with Rep; this interaction relocates Rep into the nucleus.

Subcellular Location : Host nucleus. Virion (Potential).

Database links : UniProtKB/Swiss-Prot: O56129.1

豬圓環(huán)-PCV-2是一種嗜單核-巨噬細(xì)胞性的，屬于圓環(huán)科圓環(huán)屬的成員，根據(jù)抗原性及基因組成，分為PCVl和PCV2兩種，其中PCV2對(duì)豬具有致病性，PCV2(囊膜衣殼蛋白(Cap))主要侵害，導(dǎo)致感染豬，降低機(jī)體對(duì)其他病原體的和疫苗的反應(yīng)性，容易導(dǎo)致其他和細(xì)菌的，使死亡率明顯升高。

實(shí)驗(yàn)流程：

全、新、優(yōu)、品、好四大特點(diǎn)：

豬圓環(huán)Ⅱ型衣殼蛋白費(fèi)用 全：公司提供上萬種產(chǎn)品，涵蓋了生物試劑，elisa試劑盒，標(biāo)準(zhǔn)品，培養(yǎng)基，原裝耗材，抗體、培養(yǎng)基、ATCC細(xì)胞等，基本上各種科研所需產(chǎn)品在我司都能找到。

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