抗體的生物素化標(biāo)記實(shí)驗(yàn)要點(diǎn)：

1. 磷酸化蛋白酪氨酸磷酸酶1C抗體品牌 如在反應(yīng)混合液中有疊氮鈉或游離氨基存在,會(huì)抑制標(biāo)記反應(yīng)。因此,蛋白質(zhì)在反應(yīng)前要對 0.1mol/L碳酸氫鈉緩沖液或0.5mol/L硼酸緩沖液充分透析；

2.所用的NHSB及待生物素化蛋白質(zhì)之間的分子比按蛋白質(zhì)表面的ε-氨基的密度會(huì)有所不同,選擇不當(dāng)則影響標(biāo)記的效率,應(yīng)先用幾個(gè)不同的分子比來篩選最適條件；

3.用NHSB量過量也是不利的,抗原的結(jié)合位點(diǎn)可能因此被封閉,導(dǎo)致抗體失活；

4.由于抗體的氨基不易接近可能造成生物素化不足,此時(shí)可加入去污劑如 Triton x-100, Tween20等；

5.當(dāng)游離ε-氨基(賴氨酸殘基的氨基)存在于抗體的抗原結(jié)合位點(diǎn)時(shí),或位于酶的催化位點(diǎn)時(shí),生物素化會(huì)降低或損傷抗體蛋白的結(jié)合力或活性；

6.生物素還可能與不同的功能基團(tuán),如羰基、氨基、巰基、異咪唑基及苯酚基,也可與糖基共價(jià)結(jié)合；

7.交聯(lián)反應(yīng)后,應(yīng)充分透析,否則,殘余的生物素會(huì)對生物素化抗體與親和素的結(jié)合產(chǎn)生競爭作用；

8.在細(xì)胞的熒光標(biāo)記實(shí)驗(yàn)中,中和親和素的本底低,但由于鏈霉親和素含有少量正電荷,故對某些細(xì)胞可導(dǎo)致高本底。

產(chǎn)品訂購信息：
英文名稱  Anti-phospho-PTPN6(Tyr564) 

中文名稱   磷酸化蛋白酪氨酸磷酸酶1C抗體品牌 

別    名  SHP-1(Phospho-Tyr564); PTPN6(phospho Y564); SHP1(phospho Y564); 70 kda SHP1L protein; HCP; HCPH; Hematopoietic cell phosphatase; Hematopoietic cell protein tyrosine phosphatase; Hematopoietic cell protein-tyrosine phosphatase; HPTP1C; Protein tyrosine phosphatase 1C; Protein tyrosine phosphatase non receptor type 6; Protein tyrosine phosphatase SHP1; PTP1C; PTPN6; SH PTP 1; SHP1; SHP1L; PTN6_HUMAN.

濃    度   1mg/1ml

規(guī) 格  0.1ml/100μg

抗體來源   Rabbit

克隆類型   polyclonal

交叉反應(yīng)   Human, Mouse, Rat, Dog, Pig, Horse, Sheep, Guinea Pig 

產(chǎn)品類型   一抗 磷酸化抗體  

研究領(lǐng)域    

蛋白分子量  predicted molecular weight: 65kDa

性    狀   Lyophilized or Liquid

免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human PTPN6 around the phosphorylation site of Tyr564 [V(p-Y)EN] 

亞    型   IgG

純化方法   affinity purified by Protein A

儲(chǔ) 存 液   Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

磷酸化蛋白酪氨酸磷酸酶1C抗體品牌 產(chǎn)品應(yīng)用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

（石蠟切片需做抗原修復(fù)） 

 not yet tested in other applications.

 optimal dilutions/concentrations should be determined by the end user.  

保存條件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 

Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

產(chǎn)品介紹 SHP1 is a member of the non receptor protein tyrosine phosphatase subfamily. The PTP family comprises of at least 37 proteins, characterized by a catalytic phosphatase domain of approximately 240 amino acids, and includes both transmembrane and cytosolic enzymes. PTP1B is cytosolic. The PTPs have high substrate specificity for phosphotyrosyl proteins, at the primary sequence level sharing little similarity with the protein serine phosphatases, protein threonine phosphatases, or the acid and alkaline phosphatases. SHP1 is implicated in the control of tyrosine kinase signalling pathways in cellular proliferation, with a potential role in cancer.

Function : Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.

Subunit : Monomer. Interacts with MTUS1. Interacts with MILR1 (tyrosine-phosphorylated). Interacts with KIT. Binds PTPNS1, LILRB1 and LILRB2. Interacts with FCRL2, FCRL3, FCRL4, CD300LF, CDK2 and CD84. Interacts with KIR2DL1; the interaction is enhanced by ARRB2. Interacts (via SH2 1 domain) with ROS1; the interaction is direct and promotes ROS1 dephosphorylation. Interacts with EGFR; inhibits EGFR-dependent activation of MAPK/ERK. Interacts with LYN. Interacts with the tyrosine phosphorylated form of PDPK1.

Subcellular Location : Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II. Shuttles between the cytoplasm and nucleus via its association with PDPK1.

Post-translational modifications : Phosphorylated on tyrosine residues. Binding of KITLG/SCF to KIT increases tyrosine phosphorylation. Phosphorylation at Tyr-564 enhances phosphatase activity.

Similarity : Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.

Contains 2 SH2 domains.

Contains 1 tyrosine-protein phosphatase domain.

抗體的鑒定：

1）磷酸化蛋白酪氨酸磷酸酶1C抗體品牌 抗體的效價(jià)鑒定：不管是用于診斷還是用于,制備抗體的目的都是要求較高效價(jià)。不同的抗原制備的抗體,要求的效價(jià)不一。鑒定效價(jià)的方法很多,包括有試管凝集反應(yīng),瓊脂擴(kuò)散試驗(yàn),酶聯(lián)免疫吸附試驗(yàn)等。常用的抗原所制備的抗體一般都有約成的鑒定效價(jià)的方法,以資比較。如制備抗抗體的效價(jià),一般就采用瓊脂擴(kuò)散試驗(yàn)來鑒定。

2）抗體的特異性鑒定：抗體的特異性是指與相應(yīng)抗原或近似抗原物質(zhì)的識(shí)別能力?？贵w的特異性高,它的識(shí)別能力就強(qiáng)。衡量特異性通常以交叉反應(yīng)率來表示。交叉反應(yīng)率可用競爭抑制試驗(yàn)測定。以不同濃度抗原和近似抗原分別做競爭抑制曲線,計(jì)算各自的結(jié)合率,求出各自在IC50時(shí)的濃度,并按公式計(jì)算交叉反應(yīng)率。 

如果所用抗原濃度IC50濃度為pg/管,而一些近似抗原物質(zhì)的IC50濃度幾乎是無窮大時(shí),表示這一抗血清與其他抗原物質(zhì)的交叉反應(yīng)率近似為0,即該血清的特異性較好。

3）抗體親和力：是指抗體和抗原結(jié)合的牢固程度。親和力的高低是由抗原分子的大小,抗體分子的結(jié)合位點(diǎn)與抗原決定簇之間立體構(gòu)型的合適度決定的。有助于維持抗原抗體復(fù)合物穩(wěn)定的分子間力有氫鍵,疏水鍵,側(cè)鏈相反電荷基因的庫侖力,范德華力和空間斥力。親和力常以親和常數(shù)K表示,K的單位是L/mol?？贵w親和力的測定對抗體的篩選,確定抗體的用途,驗(yàn)證抗體的均一性等均有重要意義。

LN 人層粘連蛋白Multi-class antibodies規(guī)格： 48T

Anti-CK II alpha/STK 屬絲/蘇氨酸蛋白質(zhì)激酶抗體Multi-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh NT/NTS1/NTRH 神經(jīng)降壓素抗體 規(guī)格 0.1ml

Plasmid Minipreparation Kit 質(zhì)粒小提試劑盒 100次(100個(gè)硅膠柱和試劑)

RNF125 英文名稱： 環(huán)指蛋白125抗體 0.2ml

phospho-Cofilin (Ser24) 英文名稱： 磷酸化絲切蛋白抗體 0.1ml

Anti-CK II alpha/STK 屬絲/蘇氨酸蛋白質(zhì)激酶抗體Multi-class antibodies規(guī)格： 0.2ml

IL-5 人白介素-5Multi-class antibodies規(guī)格： 48T

Anti-CGA 嗜鉻粒素A抗體Multi-class antibodies規(guī)格： 0.1ml

Rhesus antibody Rh NUP88 核孔復(fù)合體蛋白88抗體 規(guī)格 0.2ml

BCA(bicinchoninic acid) BCA蛋白濃度測定試劑盒 120ml

phospho-TCF3(Thr355) 英文名稱： 磷酸化轉(zhuǎn)錄因子3抗體 0.1ml

CQ028 英文名稱： 多癌基因下調(diào)蛋白抗體 0.2ml

Anti-CGA 嗜鉻粒素A抗體Multi-class antibodies規(guī)格： 0.1ml

Anti-LTB4-R2/FITC 熒光素標(biāo)記白三烯B4受體2抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Anti-TANK/FITC 熒光素標(biāo)記TANK抗體IgGMulti-class antibodies規(guī)格： 0.2ml

Rhesus antibody Rh ADCY4 腺苷酸環(huán)化酶4抗體 規(guī)格 0.2ml

Rabbit Anti-dog IgG/Cy5.5 Cy5.5標(biāo)記的兔抗狗IgG 0.1ml

Ghrelin Receptor 英文名稱： 腦腸肽受體抗體 0.1ml

Rhesus antibody Rh Rabbit Anti-chicken IgG/PE-Cy5.5 PE-Cy5.5標(biāo)記的兔抗雞IgG 規(guī)格 0.1ml

Anti-TANK/FITC 熒光素標(biāo)記TANK抗體IgGMulti-class antibodies規(guī)格： 0.2ml

大鼠抗單核細(xì)胞抗體(AMA)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人狀瘤病毒16型毒衣殼蛋白L1(HPV16L1)抗體(IgG)免疫試劑盒 Human papillomavirus type 16 L1-capsids(HPV16L1)aibody IgG ELISA kit

MouseIerleukin15,IL-15ELISAKit小鼠白介素15(IL-15)ELISA試劑盒規(guī)格：96T/48T

ERK5(抗人;抗兔;抗小鼠;抗大鼠)100微克

Humanmyelinprotein2,p2ELISA試劑盒人神經(jīng)髓鞘蛋白(p2)ELISA試劑盒規(guī)格：96T/48T

Humanproliferatingcellnuclearaigenaibody,PCNAELISAKit人抗增殖細(xì)胞核抗原抗體(PCNA)ELISA試劑盒規(guī)格：96T/48T

大鼠白介素1受體關(guān)聯(lián)激酶4(IRAK4)ELISA試劑盒 ，英文名： IRAK4 ELISA Kit

Human ierferon gamma (IFN- gamma) ELISA Kit 人γ干擾素(IFN-γ)ELISA試劑盒

PlaGibberellicAcid,GAELISAKit 植物赤霉素(GA)ELISA試劑盒 96T/48T 進(jìn)口分裝

CLIAKitforCK-HMW(HumancytokeratinHighMolecularWeight)ELISAKit人高分子量細(xì)胞角蛋白規(guī)格：48T/96T

細(xì)胞脂質(zhì)過氧化物(HYDROPEROXIDE)活性二甲酚橙(XYLENOLORANGE)比色法定量檢測試劑盒50次

Ratbrain-gpeptides,BGP/GehrelinELISAKit大鼠腦腸肽(BGP/Gehrelin)ELISA試劑盒規(guī)格：96T/48T

磷酸化蛋白酪氨酸磷酸酶1C抗體品牌 大鼠超敏C反應(yīng)蛋白(hs-CRP)ELISA 試劑盒 96T/48T 試劑盒 組裝/原裝

人免疫球蛋白G Fc段受體Ⅱ(FcγRⅡ/CD32)免疫試劑盒 Human Receptor Ⅱ for the Fc region of immunoglobulin G,FcγRⅡ ELISA Kit

MouseUlasensitivityThyroxine,u-T4ELISAKit小鼠高敏甲狀腺素(u-T4)ELISA試劑盒規(guī)格：96T/48T

石蠟切片組織BCL2蛋白表達(dá)NBT顯色光學(xué)顯微鏡檢測試劑盒10/20次

HumanProstaglandinDsyhase,PTGDSELISA試劑盒人素D合成酶(PTGDS)ELISA試劑盒規(guī)格：96T/48T

Humahymicsomallymphopoietin,TSLPELISAKit人胸腺基質(zhì)淋巴細(xì)胞生成素(TSLP)ELISA試劑盒規(guī)格：96T/48T